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Histo > Special Stains Pt. 1 > Flashcards

Special Stains Pt. 1 Flashcards

(67 cards)

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1
Q

Stains for lipids

A

Sudan black
Sudan IV (Scharlach R)
Oil red O
Osmic acid stain
Nile blue sulfate method
Toluidine blue-acetone method
Borohydride-PAS method

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2
Q

Stains lipids blue black

A

Sudan black

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3
Q

Stains TAG red ; most commonly used stain

A

Sudan IV (scharlach R)

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4
Q

Stains fat brilliant red

A

OIL RED O

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5
Q

Stains fat black

A

Osmic acid stain

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6
Q

Color of neutral fat in Nile blue sulfate method

A

Pinkish red

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7
Q

Color of cholesterol esters and cholesterin fatty acids in Nile blue sulfate method

A

Light red

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8
Q

Color of cerebroside in Nile blue sulfate method

A

Light blue

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9
Q

Color of fatty acids and soap in Nile blue sulfate method

A

Deep blue to violet

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10
Q

Stains sulfatide metachromatic red-brown or yellow

A

Toluidine blue-acetone method

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11
Q

Stains gangliosides red

A

Borohydride-PAS method

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12
Q

Stains for carbohydrates

A

Periodic acid Schiff (PAS)
PAS with diastase control
Langhan’s iodine (Carleton’s modif.)
Best Carmine
Fresh frozen azure A metachromatic
Metachromatic toluidine blue stain
Alcian blue
Alcian blue - PAS
Gomori’s aldehyde fuchsin stain
Mucicarmine stain
Hale’s dialyzed (colloidal) iron
Fluorescent acridine orange

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13
Q

Stains Carbohydrates, glycogen, mucins magenta red

A

Periodic Acid Schiff

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14
Q

Stains glycogen red ; stain of choice for glycogen

A

PAS with diastase control

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15
Q

Stains glycogen mahogany brown

A

Langhan’s iodine (Carleton’s modification)

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16
Q

Stains glycogen bright red ; specific for glycogen ; forms HYDROGEN BONDS

A

Best carmine

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17
Q

Stains glycosaminoglycans red purple

A

Fresh frozen azure A metachromatic
Metachromatic Toluidine blue stain

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18
Q

Stains acid mucins blue

A

Alcian blue

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19
Q

Stains acid mucin blue and neutral mucin magenta

A

Alcian blue — PAS

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20
Q

Stain sulfated mucins purple ; carboxylated mucins blue

A

Gomori’s aldehyde fuchsin stain

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21
Q

Stains mucins red

A

Mucicarmine stain

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22
Q

Stains acid mucin dark blue

A

Hale’s dialyzed (colloidal iron)

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23
Q

Stains acid mucopolysaccharides black ; stains fungi greenish red fluorescence

A

Fluorescent acridine orange

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24
Q

Stains for proteins

A

Alkaline fast - green method
Peracetic acid - Alcian blue
Sakaguchi’s test

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25
Stains histones and protamines green
Alkaline fast-green method
26
Stains cystine and cysteine blue-green
Peracetic acid - Alcian blue
27
Stains arginine orange-ref ; uses Milton reagent
Sakaguchi’s test
28
Stains for nucleic acid
Fuelgen technique Methyl-green-pyre in Acridine orange fluorescent stain
29
Stains DNA red purple ; most reliable and specific stain for DNA
Feulgen technique
30
Stains RNA (nucleoli) red and DNA (Chromatin) green
Methyl green - pyre in
31
Stains DNA yellow green fluorescent and RNA brick to orange-red fluorescence
Acridine orange fluorescent stain
32
Stains for muscle and bone
Modified Gomori’s trichrome Mallory PTAH Lissamine fast red - tartrazine Heidenhain’s iron hematoxylin Schmorl’s picro-thionine method
33
Stains fibers red and collagen green
Modified Gomori’s trichrome
34
Color muscle, neuralgia in Mallory’s PTAH
Blue
35
Color of myelin in Mallory’s PTAH
Blue to blue-gray
36
Color of collagen in Mallory’s PTAH
Reddish
37
Color of coarse elastic fibers in Mallory’s PTAH
Bluish
38
Color of fibrin in Mallory’s PTAH
Blue
39
Stains muscles and RBCs red and collagen yellow
Lissamine fast red - tartrazine
40
Stains muscle striations, mitochondria, myelin, chromatin GRAY BLACK
Heidenhain’s iron hematoxylin
41
Stains lacunae and canaliculi dark brown-black and bone matrix yellow/brownish
Schmorl’s picro-thinning method
42
Stain for connective tissues
Gomori’s silver impregnation stain Van Gieson’s stain Masson trichrome stain Mallory’s aniline blue Azocarmine Weigert’s stain Verhoeff’s stain Taezer-Unna orcein method Krajian’s technique (Congo red) Martin’s-scarlet blue Mallory’s PTAH Highman’s congo red method Alkaline Congo red method Krajian’s stain (modified bennhold) Methyl violet - crystal violet method Thioflavin-T fluorescent stain
43
Stain reticulin fibers black
Gomori’s silver impregnation stain
44
Stains collagen pink/deep red ; muscle, RBC, fibrin yellow
Van Gieson’s stain
45
Stains collagen and mucus blue ; stains muscle, RBC ,keratin red
Masson trichrome stain
46
Color of collagen fibers, fibroglia, fibrils, axon cylinders, neuroglia in Mallory’s aniline blue
Red
47
Color of elastic fibers in Mallory’s aniline blue
Pale pink/yellow
48
Color of RBC and myelin sheaths in Mallory’s aniline blue
Yellow
49
Stains connective tissue, glomerular basement membrane, amyloid, mucous colloid DEEP BLUE
AZOCARMINE
50
Stains elastic fibers dark blue/blue black
Weigert’s stain
51
Stains elastic fibers black
Verhoeff’s stain
52
Stains elastic fibers dark brown
Taenzer-Unna-Orcein method
53
Color of elastic fibers in Krajian’s technique (Congo red)
Bright red
54
Color of fibrin and connective tissue in Krajian’s technique (Congo red)
Dark blue
55
Color of RBCs in Krajian’s technique (Congo red)
Orange yellow
56
Color of collagen in Martin’s-Scarlet blue
Blue
57
Color of fibrin and muscle in Martin’s-Scarlet blue
Red
58
Color of RBCs in Martin’s-Scarlet blue
Yellow
59
Color of collagen, osteoporosis, cartilage, and elastic fibers in Mallory’s PTAH
DEEP BROWNISH RED
60
Color of fibrin, muscle striations, neuroglia, amoeba in Mallory’s PTAH
DARK BLUE
61
Color of RBCs and cilia in Mallory PTAH
Blue
62
Color of myelin in Mallory PTAH
Lighter blue
63
Stains amyloid deep pin to red
Highman’s Congo red method
64
Stains amyloid, elastic fibers, eosinophil granules RED
Alkaline Congo red method
65
Stains amyloid red on a clear background
Krajian’s stain (Modified bennhold)
66
Stains amyloid purplish red
Methyl violet - crystal violet method
67
Stains amyloid yellow fluorescence
Thioflavin-T fluorescent stain

Histo (5 decks)

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