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Histo > Special Stains Pt. 2 > Flashcards

Special Stains Pt. 2 Flashcards

(68 cards)

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1
Q

Stains for enzymes

A

Gomori calcium method
Gomori lead method
Lead method
Metal precipitation
Calcium cobalt method
Alpha-naphthyl acetate method
Indoxyl acetate (Holt & Withers)
Tetrazolium method

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2
Q

Stains ALP brownish black

A

Gomori calcium method

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3
Q

Stains ACP black ; substrate : beta-glycerophosphate

A

Gomori lead method

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4
Q

Stains 5-nucleotidase blackish brown deposits

A

Lead method

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5
Q

Stains ATPase dark brownish-black precipitate

A

Metal precipitation

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6
Q

Stains ATPase cobalt phosphate precipitate

A

Calcium cobalt method

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7
Q

Stains nonspecific esterase BLUE

A

Indoxyl acetate (Holt & Withers)

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8
Q

Stains monoamine oxidase BLUISH BLACK

A

Tetrazolium method

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9
Q

Stains for bone marrow and blood

A

Rapid Toluidine - eosin stain
Weigh-Giemsa or Jenner-Giemsa
Peroxidase reaction

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10
Q

Color of eosinophilic granule in Rapid Toluidine-eosin stain

A

Red `

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11
Q

Color of basophils, mast cell granules in Rapid Toluidine-eosin stain

A

Blue

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12
Q

Stains myeloid cells (except basophils) green to dark blue granules

A

Peroxidase reaction

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13
Q

Stain for tissue pigments and deposits

A

Perl’s Prussian blue method
Schmorl’s ferric ferricyanide
Masson-Fontana technique
Von-kossa’s silver nitrate method

Gomori’s Prussian blue
Tumbull’s blue reaction
Benzidine-nitroprusside stain
Modified fouchet’s technique
Gmelin technique
Stein’s iodine test
Gomori’s aldehyde fuchsin technique
Mallory;s fuchsin stain
Calcium -dye lake reaction
Linquist’s modified rhodamine

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14
Q

Stains hemosiderin DEEP BLUE

A

Perl’s Prussian blue method

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15
Q

Stains iron pigments DEEP BLUE

A

Gomori’s Prussian blue

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16
Q

Stains ferrous iron (hemosiderin) BLUE

A

Tumbull’s blue reaction

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17
Q

Stains hemoglobin and oxidase granules DARK BLUE

A

Benzidine - nitroprusside stain

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18
Q

Stains bile pigments EMERALD TO BLUE GREEN

A

Modified fouchet’s technique

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19
Q

Stains bile and hematoidin BLUE PURPLE THEN GREEN

A

Gmelin technique

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20
Q

Stain for bile pigments

A

Stein’s iodine test

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21
Q

Stains bile, LIPOFUCHSIN, melanine, ARGENTAFFIN CELL, chromatin, thyroid colloid BLUE

A

Schmorl’s ferric ferricyanide

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22
Q

Stains lipofuscin PURPLE

A

Gomori’s aldehyde fuchsin technique

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23
Q

Stains hemofuscin RED

A

Mallory’s fuchsin stain

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24
Q

Stains melanin and ARGENTAFFIN CELL granules BLACK

A

Masson-Fontana technique

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25
Stains calcium salts INTENSE REDDISH ORANGE
Calcium-dye lake reaction
26
Stains calcium BLACK
Von kossa’s silver nitrate method
27
Stains copper and copper-associated protein RED TO ORANGE-RED
Lindquist modified rhodamine
28
Stains for CNS tissues
Bielchowsky’s technique Luxor fast blue - H&E stain Kluver and Barrera Luxol fast blue Toluidine blue Polychrome methylene blue Thionine Cresyl fast violet stain Bodian’s stain Sexier - Munger technique Luxol fast blue -PAS stain Weil’s method Cajal’s gold sublimate method Modified Holzer’s method
29
Stains NEUROFIBRIL, axons, and dendrites BLACK ON A GRAYISH BACKGROUND
Bielschowsky’s technique
30
Stains myelin BLUE GREEN
LUXOL FAST BLUE - H&E STAIN Kluver & Barrer;s Luxol fast blue
31
Stains Nissl granules (aka Tigroid substances) DEEP BLUE
Toluidine blue
32
Stains Nissl granules PURPLE
THIONINE
33
Stains Nissl granules PURPLE-DARK BLUE ; stains neurons PALE PURPLE BLUE
Cresyl fast violet stain
34
Stain for nerve fibers, nerve endings, and diagnosis of Alzheimer’s disease
Bodian’s stain
35
Color of myelin sheath in Sevier-Munger technique
Light brown
36
Color of peripheral neuritis, axons, neuritic plaques, tangles, ARGENTAFFIN granules in Sevier-Munger technique
BLACK
37
Stains myelin sheath BLUE BLACK
Weigert-Pal technique
38
Color of myelin in luxol fast blue - PAS stain
Blue-green
39
Color of fungi & PAS (+) elements in luxol fast blue - PAS stain
Rose to red
40
Color of cytoplasmic nucleoproteins in luxol fast blue - PAS stain
Bluish purple
41
Color of capillaries in luxol fast blue - PAS stain
Red
42
Stains myelin BLACK
Weil’s method
43
Color of astrocytes in Cajal’s gold sublimate method
Black
44
Color of nerve cells in Cajal’s gold sublimate method
Red
45
Color of nerve fibers in Cajal’s gold sublimate method
Unstained
46
Stains glial fibril BLUE
Modified Holzer’s method
47
Stains for microorganism
Wade-Fite technique Levaditi’s method Warthin-Starry method Grocott Methenamine silver Orcein method Ziehl-Nielsen method Auramine Rhodamine Toluidine blue stain Cresyl violet acetate method Dieterle method Gram-Twort stain Brown & Brenn method Modified Steiner and Steiner Lendrum’s phloxine-tartrazine Rapid Giemsa stain
48
Stains M. Leopard other mycobacteria and Nocardia RED
Wade-Fite technique
49
Stains AFB RED
Ziehl-Neelsen method
50
Stains mycobacteria GOLDEN YELLOW FLUORESCENCE
Auramine Rhodamine
51
Stains H pylori DARK BLUE AGAINS BLUE BACKGROUND
Toluidine blue stain
52
Stains spirochetes BLACK ON A YELLOWISH BACKGROUND
Levaditi’s method
53
Stains spirochetes BLACK
Warthin-Starry method
54
Color of mucin, glycogen, and fungi in Grocott Methenamine silver
Black
55
Color of mycelia and hyphae in Grocott Methenamine silver
Old rose
56
Color of RBCs in Grocott Methenamine silver
Yellow
57
Stains HBsAg and some mucins BROWN BLACK
Orcein method
58
Stains gram positive BLUE BLACK
GRAM-TWORT STAIN
59
Stains gram negative PINK-RED
GRAM-TWORT STAIN
60
Color of gram positive in Brown and Brenn method
Blue
61
Color of gram negative in Brown and Brenn method
Red
62
Stains H pylori BLUE-VIOLET
Cresyl violet acetate method
63
Stains L pneumophila and spirochetes DARK BROWN TO BLACKC
Dieterle method
64
Stains spirochetes, Donovan bodies, fungi, bacteria BLACK
MODIFIED STEINER AND STEINER
65
Stains viral inclusions BRIGHT RED
Lendrum’s phloxine - tartrazine
66
Color of bacteria in rapid giemsa stain
Blue
67
Color of mast cell granules in rapid giemsa stain
Deep blue
68
Color of eosinophil granules in rapid giemsa stain
Red

Histo (5 decks)

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