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Clinical Chemistry > Specimen Collection And Handling > Flashcards

Specimen Collection And Handling Flashcards

(75 cards)

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1
Q

Tube used for finger sticks use tubes with the smallest volume necessary for testing to prevent iatrogenic anemia/hospital acquired anemia

A

Lancets

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2
Q

Tube used for coagulation studies (PT or PTT)

A

Light blue or blue

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3
Q

Additives of light blue top

A

Sodium citrate
Thrombin and soybean trypsin inhibitor

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4
Q

Test affected by additive carry over in light blue top

A

Citrate
ALP
Calcium
Phosphorus

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5
Q

Tube used in routine chemistries, therapeutic drug levels, immunohematology, and serology

A

Red (Glass)

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6
Q

Additive of Red (plastic) tube

A

Clots activator (silica)

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7
Q

Test affected by additive carry over in red (plastic)

A

Silica
Partial thromboplastin time
Protime

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8
Q

Additive of Red/Black (tiger) Gold Red/Gold tube

A

Clot activator and gel separator

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9
Q

Additive of Green/Gray Light green tube

A

Lithium Heparin and Gel separator

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10
Q

Tube used for routine and special chemistry and cytogenetic testing

A

Green/Gray Light green

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11
Q

Additive of Green tube

A

Lithium heparin
Sodium Heparin

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12
Q

Test affected by additive carry over

A

Heparin
Activated clotting time
ACP
Calcium
Sodium
Lithium
Partial thromboplastin
Protime

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13
Q

Best anticoagulant for hemolysis prevention and for STAT sample

A

Heparin

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14
Q

Tube used in hematology for complete blood count, slide preparation, routine hematology procedures\ immunohematology and some special chemistry assays

A

Lavender or pink top

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15
Q

Additives of Lavender or pink top

A

EDTA/thylenediaminetetraacetate

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16
Q

Tests affected by additive carry over

A

EDTA
ALP
Ca
K
Fe
Sodium
CK
Partial thromboplastin
Protime

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17
Q

EDTA when used in electrolytes will increase:

A

Sodium and potassium

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18
Q

EDTA when used in electrolytes will decrease:

A

Calcium and Fe

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19
Q

Tube used to inhibit glycolysis

A

Gray top

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20
Q

Additive of gray top

A

Sodium fluoride and Potassium oxalate
Sodium Fluoride and EDTA
Sodium Fluoride

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21
Q

Additives that prevent fermentation of glucose

A

Sodium fluoride and Potassium oxalate
Sodium Fluoride and EDTA
Sodium Fluoride

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22
Q

Distorts RBC morphology

A

Oxalates and sodium fluoride

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23
Q

Tests affected by additive carry over

A

Oxalates
ACP and ALP
Ca
K
LD
Partial thromboplastin
Protime
Sodium Fluoride
Na

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24
Q

Additive of Orange Gray/Yellow top

A

Thrombin

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25
Tube used in chemistry and toxicology
Royal blue top
26
Additives of royal blue top
None (red label) EDTA (Lavender label) Sodium Heparin (Green label)
27
Additives of Tan (glass tube) and Tan (plastic) Yellow tube
Sodium Heparin EDTA
28
Additives of yellow tube
Sodium Polyanethol sulfonate (SPS) Acid Citrate Dextrose (ACD)
29
Tube used in WBC preservative
Yellow tube
30
Tubes w/gel are not used for:
ImmunoSero Blood bank Trace Metals TDM
31
Tube colors with EDTA:
Lavender (glass and plastic) Pink White Royal blue Tan (plastic)
32
Tubes that chelates calcium
EDTA Sodium citrate All tubes with EDTA + blue, light blue
33
Tubes that inhibit thrombin formation
Lithium Heparin Sodium Heparin Light green Green Black Royal blue
34
Tubes with clot activator
Red (plastic/hemogard) (Silica) Red/gray and gold (Silica) Yellow/Gray and orange
35
Tube that acts fibrin degradation product
Light blue
36
Tube used for blood bank, microbiology, HLA phenotyping and Paternity testing
Yellow tube
37
Order vein choice
Median Cephalic Basilic
38
Anchored and less painful vein
Median
39
Vein common in obese patients
Cephalic
40
Vein near cutaneous nerve and brachial artery and painful
Basilic
41
These are symptoms of nerve puncture:
Electric shock Arm numbness Burning Pain radiating up or down
42
Problem sites in venipuncture
Burn, scars or tattooed Damaged veins Edema Hematoma Mastectomy Lymph removed
43
It inspects an organ by passing light thru its wall LED or infrared light
Transillumination
44
If blood pressure cuff used in venipuncture
60 mmHg
45
Angle in venipuncture
15-30 degrees
46
Angle of deep vein
High angle
47
Angle of not too deep vein
Low angle
48
It introduces a tissue factor then factor 7 activates then PT will shorten. Possible nerve damage.
Probe fishing
49
Length of needle in venipuncture
1 or 1.5 inches
50
Antiseptic for ethanol testing
Benzalkonium chloride
51
Standard needle for venipuncture
21 gauge
52
Causes of collapse vein
Too much vacuum Quick pull of plunger Tight tourniquet or close to the site of puncture
53
Order of draw of venipuncture
Blood culture Citrate Red (glass) Red (plastic) Heparin EDTA Sodium fluoride
54
The most common cause of carry over
EDTA
55
The transfer of one additive/specimen to another thru needle contact causing contamination
Carry over
56
It means to detect errors and to minimize clerical errors
Bar code labels
57
Postphlebotomy Neurologic complications
Diaphoresis Seizure Pain Nerve damage
58
Most common complication in blood
Ecchymosis (Bruise)
59
In pediatric venipuncture, how many percent of loss of blood that can lead to shock and cardiac arrest
10%
60
Arterial puncture is used for:
Blood gas and pH
61
Test conducted prior to collection in arterial puncture
Modified Allen test
62
Unacceptable sites in arterial puncture
Irritated, edematous, near a wound, or area with fistula
63
Length of lancet in skin puncture
1.75mm
64
Depth of incision in skin puncture
<2.0mm in infants <2.5mm in adults
65
Preferred sites in skin puncture
Lateral plantar heel (newborn) 3rd and 4th fingers Earlobes (least site)
66
Order of filling microtainer
Blood gas analysis Slides EDTA Other tubes with additives Non-additive tubes
67
Used for measuring pH and pCO2 but not pO2
Arterialized capillary blood
68
Preferred site of arterialized capillary blood
Earlobes
69
In arterialized capillary blood, don't perform if arterial BP is below:
95 mmHg
70
Often created when multiple tests are ordered on a single specimen.
Aliquot
71
In case of delay, most samples are refrigerated at:
4°C for 8 hrs
72
Cell lysis and slight elevation of LD and K
Excessive centrifugation
73
Causes of hemolysis
Too small needle Fast pulling of plunger Fast transfer of blood to tube Shaking tube vigorously Blood collected before alcohol dried Rimming
74
Causes hemolysis and aerosol infection
Rimming
75
Any test used as evidence in legal proceedings; e.g., blood alcohol, drug screens, DNA analysis. Procedure to guarantee integrity of specimens to court, e.g., legal blood alcohol, drug test.
Chain of custody

Clinical Chemistry (6 decks)

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