Stem Cell Therapy Flashcards Preview

Liz's Blood Bank Module 2 > Stem Cell Therapy > Flashcards

Flashcards in Stem Cell Therapy Deck (18):
1

Stem cell marker for hematopoietic stem cells

CD34

2

List sources of hematopoietic stem cells for transplantation

- BM
- PB progenitor cells
- Cord blood

3

3 types of stem cell transplants

- Autologous
- Allogeneic
- Syngeneic

4

If a patient serves as their own donor, this would be a ____ stem cell transplant

Autologous

5

If a volunteer donor provides stem cells for a recipient that is an HLA match, this would be a ____ stem cell transplant

Allogeneic

6

If a patient's identical twin were donate their stem cells, this would be a ____ stem cell transplant

Syngeneic

7

List the diseases treatable w/ stem cell transplants

- Germ cell tumors
- Neuroblastoma
- Amyloidosis
- MDS
- Aplastic anemia

8

Hematopoietic stem cell processing steps

- Flow cytometry
- Cryopreservation
- Concentration
- RBC depletion
- Thaw/wash
- Filtering
- Infusion of thawed HSCs

9

Flow cytometry procedure

- Detects and enumerates stem cells
- Fluorescent tags product sample w/ anti-CD45 and anti-CD34 to determin stem cell content in HSC product
- Physician determines apheresis collection status

10

Cryopreservation procedure

- Controlled rate freezer slowly cools cells to -90°C at a rate of 1°C/minute to prevent ice crystal formation
- Cryprotectant DMSO is used to maintain HSC viability
- Frozen cells are stored: vapor phase liquid nitrogen (LN2) freezer at < -150°C
- Able to store for up to 12 years

11

Cell concentration procedure

Uses centrifugation to reduce volume by plasma depletion w/ minimal WBC loss

12

RBC depletion procedure

Minimizes exposure to RBC for allogeneic recipients w/ ABO mismatch

13

Thaw/Wash procedure

- Cells are thawed/washed; dextran 40 and 5% HSA (in the lab) to remove DMSO before infusion
- Routinely performed on cord bloods to minimize pediatric infusion reaction

14

Filtering procedure

- Harvested BM is filtered to remove spicules and clots
- 1-3 bags are collected and pooled into 1 bag for transport
- Infused fresh

15

Thawed HSC infusion procedure

- Performed at patient's bedside
- Cells are transported from storage freezer to patient bedside via portable LN2 shipper
- Cells thawed in 40°C saline waterbath
- Cells infused immediately to minimize DMSO exposure and prevent cell death via central intravenous line
- Only saline is infused simultaneously

16

List the typical reactions to stem cell infusions

- Chills
- Coughing
- Chest pain (tightness)
- Redness in face (flushing)
- Nausea and/or vomiting

17

Primary method for determining success of a stem cell transplant

Engraftment

18

What is the primary cause of the reactions to stem cell infusions?

Reaction to cryoprotectant DMSO adn resolve post infusion