Component Preparation Flashcards Preview

Liz's Blood Bank Module 2 > Component Preparation > Flashcards

Flashcards in Component Preparation Deck (47):
1

What is the role of ATP and 2,3-DPG as it pertains ot RBC survival

- ATP helps RBCs move Na+ and Ca2+ out of the cell
- 2,3-DPG controls hemoglobin affinity for O2

2

State the primary goal of a RBC perservative

To provide 75% survival of transfused blood 24 hours post-transfusion

3

Changes seen in stored blood
- Plasma K+

INCREASED

4

Changes seen in stored blood
- Plasma Na+

DECREASED

5

Changes seen in stored blood
- Plasma hemoglobin

INCREASED

6

Changes seen in stored blood
- ATP

DECREASED

7

Changes seen in stored blood
- 2,3-DPG

DECREASED

8

Changes seen in stored blood
- pH

DECREASED

9

Expiration date of blood drawn in ACD and CPD

21 days

10

Expiration date of blood drawn in CPDA

35 days

11

Expiration date of blood drawn in AdSol

42 days

12

Centrifugation process when preparing packed RBCs

?

13

Centrifugation process when preparing random platelets

?

14

Centrifugation process of fresh frozen plasma

?

15

Centrifugation process of cryoprecipitate

WB → hardspin → plt-poor plasma → freeze plasma → thaw plasma in 1-6°C waterbath → "Cryo" will precipitate in cold → hardspin → remove cryo-poor plasma, leaving cryo precipitate in the bag

16

Difference b/w components prepared in an "open system" and "closed system"

?

17

Expiration date w/ an open system

24 hours

18

Expiration date w/ a closed system

Does not change

19

Describe the use of a "sterile docking device"

?

20

Give examples of when a "sterile docking device" is used

?

21

How does the procedure of a "sterile docking device" affect the expiration of the product?

?

22

Why do platelet products need constant agitation?

To prevent them from clumping

23

How do irradiation of products prevent Graft vs. Host Disease (GVHD)?

Irradiation destroys T lymphocyte's ability to mount an immune response

24

Calculate the enw expiration date when red cell or platelet products are irradiated

?

25

Proper packing procedure when shipping Red cell products (liquid)

Ship w/ "wet ice" or crushed ice, not touching donor units (separated by cardboard)

26

Proper packing procedure when shipping platelet products

Ship at room temperature

27

Proper packing procedure when shipping frozen platelet products (FFP, Cryo, frozen cells)

Ship w/ dry ice

28

Packed Cells, AdSol
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 42 days
- Storage temp: 1-6°C
- Content: RBCs, WBC, ↓ plasma
- QC: ≤ 80% Hct
- Indications: Anemia
- Tx criteria: XM required; ABO-compatible required

29

Leukoreduced packed cells
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 42 days
- Storage temp: 1-6°C
- Content: RBCs, ↓ plasma
- QC: ≤ 80% Hct, < 5x10^6 WBCs
- Indications: Anemia, hx of febrile rxns
- Tx criteria: XM required; ABO-compatible required

30

Washed packed cells
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 24 hours
- Storage temp: 1-6°C
- Content: RBCs
- QC: < 5 x 10^6 WBCs
- Indications: Anemia, IgA deficient patients, removal of K+ and anticoagulant
- Tx criteria: XM required; ABO-compatible required

31

Frozen cells/Deglyc'd cells
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 10 years frozen/24 hours thawed
- Storage temp: ≤ -65°C frozen/1-6°C thawed
- Content: RBCs
- QC: < 5 x 10^6 WBCs
- Indications: Anemia, IgA deficient patients, rare units
- Tx criteria: XM required; ABO-compatible required

32

Random platelets
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 5 days
- Storage temp: RT w/ agitation
- Content: Plt, plasma, WBCs
- QC: ≥ 5.5 x 10^10 plts; ≥ 6.2 pH
- Indications: Low platelets
- Tx criteria: ABO-compatible preferred

33

Random platelet, pool
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 4 hours
- Storage temp: RT w/ agitation
- Content: Plt, plasma, WBCs
- QC: ≥ 6.2 pH
- Indications: Low plts
- Tx criteria: ABO-compatible preferred

34

Apheresis platelets
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 5 days
- Storage temp: RT w/ agitation
- Content: Plt, plasma, WBCs
- QC: ≥ 3.0 x 10^11 plts; ≥ 6.2 pH
- Indications: Low plts
- Tx criteria: ABO-compatible preferred

35

AFFP, FFP, PF24, frozen
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 1 year
- Storage temp: ≤ -18°C
- Content: Coagulation clotting factors, plasma
- QC: N/A
- Indications: Coagulation factors, liver disease, DIC
- Tx criteria: ABO-compatible preferred

36

PF24, FFP, thawed
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 5 days
- Storage temp: 1-6°C
- Content: stable coag factors
- QC: N/A
- Indications: Stable factors, liver disease, DIC
- Tx criteria: ABO-compatible preferred

37

AFFP, thawed
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 24 hours
- Storage temp: 1-6°C
- Content: Labile and stable factors
- QC: N/A
- Indications: All coag factors, liver disease, DIC
- Tx criteria: ABO-compatible preferred

38

Cryo, frozen
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 1 year
- Storage temp: ≤-18°C
- Content: Factor VIII, vWF, XIII, fibrinogen
- QC: ≥ 80 IU VIII; ≥ 150 mg fibrinogen
- Indications: Hemophilia A, vW disease, XIII deficiency, fibrinogen deficiency, fibrin glue
- Tx criteria: ABO compatible preferred

39

Cryo, thawed
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 6 hours
- Storage temp: Room temperature
- Content: Factor VIII, vWF, XIII, fibrinogen
- QC: ≥ 80 IU VIII; ≥ 150 mg fibrinogen
- Indications: Hemophilia A, vW disease, XIII deficiency, fibrinogen deficiency, fibrin glue
- Tx criteria: ABO compatible preferred

40

Cryo, thawed and pooled
- Shelf life
- Storage temp
- Content
- QC
- Indications
- Tx criteria

- Shelf life: 4 hours
- Storage temp: Room temperature
- Content: Factor VIII, vWF, XIII, fibrinogen
- QC: ≥ 80 IU VIII; ≥ 150 mg fibrinogen
- Indications: Hemophilia A, vW disease, XIII deficiency, fibrinogen deficiency, fibrin glue
- Tx criteria: ABO compatible preferred

41

List the anticoagulants that play a part in the survival of donor blood

- Trisodium citrate
- Citric acid
- Dextrose
- Na phosphate
- Adenine
- Mannitol

42

What is the function of the anticoagulant trisodium citrate?

Binds Ca2+ and prevents clotting

43

What is the function of the anticoagulant citric acid?

Maintains pH

44

What is the function of the anticoagulant dextose

Provides energy source

45

What is the function of the anticoagulant Na phosphate?

Maintains 2,3-DPG levels and ↑ pH

46

What is the function of the anticoagulant adenine?

↑ ADP levels which results in synthesis of more ATP

47

What is the function of the anticoagulant mannitol?

Protects against spotnaneous storage hemolysis