Component Preparation Flashcards
What is the role of ATP and 2,3-DPG as it pertains ot RBC survival
- ATP helps RBCs move Na+ and Ca2+ out of the cell
- 2,3-DPG controls hemoglobin affinity for O2
State the primary goal of a RBC perservative
To provide 75% survival of transfused blood 24 hours post-transfusion
Changes seen in stored blood
- Plasma K+
INCREASED
Changes seen in stored blood
- Plasma Na+
DECREASED
Changes seen in stored blood
- Plasma hemoglobin
INCREASED
Changes seen in stored blood
- ATP
DECREASED
Changes seen in stored blood
- 2,3-DPG
DECREASED
Changes seen in stored blood
- pH
DECREASED
Expiration date of blood drawn in ACD and CPD
21 days
Expiration date of blood drawn in CPDA
35 days
Expiration date of blood drawn in AdSol
42 days
Centrifugation process when preparing packed RBCs
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Centrifugation process when preparing random platelets
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Centrifugation process of fresh frozen plasma
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Centrifugation process of cryoprecipitate
WB → hardspin → plt-poor plasma → freeze plasma → thaw plasma in 1-6°C waterbath → “Cryo” will precipitate in cold → hardspin → remove cryo-poor plasma, leaving cryo precipitate in the bag
Difference b/w components prepared in an “open system” and “closed system”
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Expiration date w/ an open system
24 hours
Expiration date w/ a closed system
Does not change
Describe the use of a “sterile docking device”
?
Give examples of when a “sterile docking device” is used
?
How does the procedure of a “sterile docking device” affect the expiration of the product?
?
Why do platelet products need constant agitation?
To prevent them from clumping
How do irradiation of products prevent Graft vs. Host Disease (GVHD)?
Irradiation destroys T lymphocyte’s ability to mount an immune response
Calculate the enw expiration date when red cell or platelet products are irradiated
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