Test 2 COPY Flashcards
(25 cards)
1
Q
steps to focusing a microscope
A
- scanning lens (4x) and coarse adjustment knob to find specimen
- fine focus the sample with fine adjustment knob
- move to low power (10x)
- fine adjust
- move to medium power (40x)
- fine adjust
2
Q
steps when using immersion oil
A
- move 1/2 way to 100x lens
- add immersion oil to lens
- move 100x lens all the way
- fine focus
3
Q
how to clean and store microscope
A
- remove slide
- turn to scanning lens
- turn off light
- unplug and wrap cord around base
- clean oil immersion and high power lens with lens paper and cleaner
- replace cover and return to cabinate with the arm facing out
4
Q
total magnification is determined by
A
objective x ocular lens
5
Q
scanning lens
A
red
4x
6
Q
low power lens
A
yellow
10x
7
Q
high power lens
A
blue
40x
8
Q
oil immersion lens
A
white
100x
9
Q
ocular lens
A
10x
10
Q
diplo
A
pair
11
Q
strepto
A
chains
12
Q
staphylo
A
chains
13
Q
steps for a smear
A
- thin film of water and bacteria
- air dry
- heat fix
14
Q
gram stain dyes
A
crystal violet
safranin
15
Q
acid fast stain dyes
A
carbolfuchsin
methylene blue
16
Q
endospore stain dyes
A
malachite green
safranin
17
Q
gram positive
A
purple
18
Q
gram negative
A
red/pink
19
Q
acid fast positive
A
red
20
Q
acid fast negative
A
blue
21
Q
endospore positive
A
green
22
Q
endospore negative
A
red
23
Q
bacteria positive in acid fast
A
mycobacteria
24
Q
diseases of bacteria positive in acid fast
A
TB
MAC disease
encephalitis
25
bacteria positive in endospore stain
bacillus and clostridium
| ex. botulism, anthrax, tetanus
