Test 2 COPY Flashcards Preview

Micro Lab Tests > Test 2 COPY > Flashcards

Flashcards in Test 2 COPY Deck (25):
1

steps to focusing a microscope

- scanning lens (4x) and coarse adjustment knob to find specimen
- fine focus the sample with fine adjustment knob
- move to low power (10x)
- fine adjust
- move to medium power (40x)
- fine adjust

2

steps when using immersion oil

- move 1/2 way to 100x lens
- add immersion oil to lens
- move 100x lens all the way
- fine focus

3

how to clean and store microscope

- remove slide
- turn to scanning lens
- turn off light
- unplug and wrap cord around base
- clean oil immersion and high power lens with lens paper and cleaner
- replace cover and return to cabinate with the arm facing out

4

total magnification is determined by

objective x ocular lens

5

scanning lens

red
4x

6

low power lens

yellow
10x

7

high power lens

blue
40x

8

oil immersion lens

white
100x

9

ocular lens

10x

10

diplo

pair

11

strepto

chains

12

staphylo

chains

13

steps for a smear

1. thin film of water and bacteria
2. air dry
3. heat fix

14

gram stain dyes

crystal violet
safranin

15

acid fast stain dyes

carbolfuchsin
methylene blue

16

endospore stain dyes

malachite green
safranin

17

gram positive

purple

18

gram negative

red/pink

19

acid fast positive

red

20

acid fast negative

blue

21

endospore positive

green

22

endospore negative

red

23

bacteria positive in acid fast

mycobacteria

24

diseases of bacteria positive in acid fast

TB
MAC disease
encephalitis

25

bacteria positive in endospore stain

bacillus and clostridium
ex. botulism, anthrax, tetanus