Test 4 Flashcards
(39 cards)
psychrophile
cold loving
mesophile
body temp loving
thermophile
heat loving
what are the bacterial susceptibility and resistance levels
resistant
intermediate
sensitive
catalase shows presence of
enzyme that converts toxic h202 to o2 and water
catalase is seen in
aerobe and facultative anaerobes
enterococcus is catalase
negative
streptococcus is catalase
negative
catalase positive
bubbling
why is catalase test performed
differentiate between streptococci and staphylococci
oxidase tests for presence of
cytochrome c oxidase in the ETC
oxidase positive
blueish purple
oxidase negative
no color or faint beige/pink
oxidase differentiates
aerobic, gram-neg bacilli from facultative anaerobic, gram neg bacilli
PEA is
phenylethyl alcohol agar
PEA interferes with
gram neg bacteria preventing their growth - selects for gram pos
PEA positive
growth - indicates gram positive
Sheep’s blood agar
SBA
not selective
tests for hemolysis
beta hemolysis
complete hemolysis
clear zones
alpha hemolysis
incomplete hemolysis
greenish halo
gamma hemolysis
no lysis
growth with no impact to agar
mannitol salt agar
MSA
selective for halophiles because has high salt concentration
differentiates between those that do and don’t ferment mannitol
msa often used for growth of
staphylococcus, micrococcus, enterococcus
MSA with growth and red
halophile
does not ferment mannitol