Test 4 Flashcards Preview

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Flashcards in Test 4 Deck (39):
1

psychrophile

cold loving

2

mesophile

body temp loving

3

thermophile

heat loving

4

what are the bacterial susceptibility and resistance levels

resistant
intermediate
sensitive

5

catalase shows presence of

enzyme that converts toxic h202 to o2 and water

6

catalase is seen in

aerobe and facultative anaerobes

7

enterococcus is catalase

negative

8

streptococcus is catalase

negative

9

catalase positive

bubbling

10

why is catalase test performed

differentiate between streptococci and staphylococci

11

oxidase tests for presence of

cytochrome c oxidase in the ETC

12

oxidase positive

blueish purple

13

oxidase negative

no color or faint beige/pink

14

oxidase differentiates

aerobic, gram-neg bacilli from facultative anaerobic, gram neg bacilli

15

PEA is

phenylethyl alcohol agar

16

PEA interferes with

gram neg bacteria preventing their growth - selects for gram pos

17

PEA positive

growth - indicates gram positive

18

Sheep's blood agar

SBA
not selective
tests for hemolysis

19

beta hemolysis

complete hemolysis
clear zones

20

alpha hemolysis

incomplete hemolysis
greenish halo

21

gamma hemolysis

no lysis
growth with no impact to agar

22

mannitol salt agar

MSA
selective for halophiles because has high salt concentration
differentiates between those that do and don't ferment mannitol

23

msa often used for growth of

staphylococcus, micrococcus, enterococcus

24

MSA with growth and red

halophile
does not ferment mannitol

25

MSA with growth and yellow

halophile
ferments mannitol

26

MSA with no growth

not halophile

27

MSA differentiates between

S. aureus and S. epidermidis

28

MacConkey agar

MAC agar
selective for gram negative
differential for lactose fermentation

29

MAC agar with pink growth

gram negative
ferments lactose

30

MAC agar with colorless growth

gram negative
does not ferment lactose

31

no growth on MAC agar

gram positive

32

eosin methylene blue agar

EMB agar
selective for gram neg
differential for fermentation of lactose and or sucrose

33

EMB with green metallic

gram neg that ferments either sugar

often E.coli

34

EMB with colorless growth

gram neg
does not ferment lactose or sucrose

35

coagulase test checks for

the formation of a clot in a tube of rabbit plasm indicating that the bacterium produces coagulase to convert fibrinogen into fibrin

36

coagulase differentiates between

s. aureus from others

37

latex agglutination

tests for specific antigens by mixing with latex bead coated with a specific antibody

38

latex aggluntination positive

clumping

39

enteropluri identifies

enterobacteriacea