tests Flashcards
(5 cards)
how to test for protein
-add small amount of sample to test tube
-add Biuret solution
- shake / mix
- if protein present = goes purple/ lilac
-if protein not present = remains blue
Test for starch
-add iodine solution to food sample
-if starch is present : goes blue-black
-if no starch : remains orange -brown
Test for reducing sugars
- add Benedict’s solution to sample
-heat in water bath for 5 mins
-if sugar present: solution turns green/ yellow/ orange / brick red
-if sugar not present it remains blue
Test for lipids
-add ethanol and shake
-pour into cold water
-if lipids present : cloudy white emulsion form
-if lipids not present: solution remains clear
experiment to show how different concentrations of sodium chloride solution affect appearance of plant cells
immerse onion skin (onion epidermis)
same volume of solutions
at least 2 different concentrations of salt solution
leave cells for 2 minutes
observe/ photograph under microscope