TESTS FOR ENTEROBACTERIACEAE Flashcards
1
Q
Phenol red
FeSO4
A
TSI
Yellowing = fermentation Splitting = gas Blackening = H2S
2
Q
Bromcresol purple
A
LIA AND DECARBOXYLASE
Decarboxylation: Purple (- ) Yellow
Deamination: red (-) PURPLE
DECARBOXYLASE: PURPLE
3
Q
Kovac’s reagent
A
Indole
Red ring
4
Q
Methyl red
A
MR
red (-) Yellow
5
Q
40% KOH + αnaphthol
A
VP
red (-) Yellow
6
Q
Bromthymol blue
A
Citrate AND AMA
BLUE ON BOTH TESTS
(-) GREEN/YELLOW
7
Q
Phenol red
A
Urease
Pink (-) Yellow
8
Q
ONPG
A
ONPG
Yellow
9
Q
10% ferric chloride
A
PAD
green
10
Q
Decarboxylation of lysine,
ornithine, arginine
A
Decarboxylase
11
Q
Initial ID of Proteus, Providencia
and Morganella
A
PAD
12
Q
ID late lactose fermenters
A
ONPG
13
Q
Urease production
A
Urease
14
Q
Acetate, malonate, acetamide as
source of C
A
AMA
15
Q
Citrate as source of carbon
A
Citrate
