Transcription Flashcards
(7 cards)
Transcription - Step 1
DNA in region of a gene unwinds & unzips
Nucleotide on both sides exposed
Transcription - Step 2
One strand is used to direct synthesis of mRNA (U replaces T) - template strand
Other strand, same sequence as mRNA (T bases not U) - complimentary strand
Transcription - Step 3
Promoter - Particular nucleotide sequence at beginning of a gene, signals the start of a gene
Proteins position RNA polymerase on DNA to bind with promoter
Transcription - Step 4
Complimentary RNA nucleotides joined together by RNA polymerase moving along length of DNA
Base sequence at end of gene = stop signal, mRNA released as single strand
Transcription - Step 5
DNA zips up & twists itself into double helix again once mRNA has peeled off
Transcription - Step 6
Intron - section of DNA that doesn’t encode a protein sequence
Exons - coding regions, contain info for protein formation
Introns spliced before mature mRNA leaves nucleus
Transcription - Step 7
Mature mRNA ready to leave nucleus and move to cytoplasm, where translation of nuclear code is carried out by ribosomes