Unit 1 DNA replication Flashcards

1
Q

3’ - 5’

A

Strand of nucleic acid running from a sugar to a phosphate

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2
Q

DNA polymerase

A

Enzyme that adds free complementary DNA nucleotides during replication of DNA

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3
Q

Heat tolerant DNA polymerase

A

Enzyme from hot-spring bacteria, used in PCR

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4
Q

Lagging strand

A

DNA strand that is replicated in fragments

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5
Q

Leading strand

A

DNA strand that is replicated continuously

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6
Q

Ligase

A

Enzyme that joins DNA fragments to complete the lagging strand

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7
Q

Polymerase chain reaction (PCR)

A

Method of amplifying sequences of DNA in vitro

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8
Q

Primer

A

Short complementary strand of DNA, start point for Polymerase

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9
Q

Replication

A

Formation of identical copies of DNA molecules

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10
Q

Template strand

A

DNA strand on which a complementary copy is built up

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11
Q

3’ end

A

End of DNA starand with sugar

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12
Q

5’ end

A

End of DNA with Phosphate group

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13
Q

Strand of nucleic acid running from a sugar to a phosphate

A

3’ - 5’

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14
Q

Enzyme that adds free complementary DNA nucleotides during replication of DNA

A

DNA polymerase

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15
Q

Enzyme from hot-spring bacteria, used in PCR

A

Heat tolerant DNA polymerase

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16
Q

DNA strand that is replicated in fragments

A

Lagging strand

17
Q

DNA strand that is replicated continuously

A

Leading strand

18
Q

Enzyme that joins DNA fragments to complete the lagging strand

A

Ligase

19
Q

Method of amplifying sequences of DNA in vitro

A

Polymerase chain reaction (PCR)

20
Q

Short complementary strand of DNA, start point for Polymerase

A

Primer

21
Q

Formation of identical copies of DNA molecules

A

Replication

22
Q

DNA strand on which a complementary copy is built up

A

Template strand

23
Q

End of DNA starand with sugar

A

3’ end

24
Q

End of DNA with Phosphate group

A

5’ end