Unit 6 Collumn 1 Flashcards
Transformation Experiment
an experiment that demonstrates the process of bacteria taking up and incorporating external genetic material (DNA) from their environment
Plasmid
a small
Hershey and Chase Experiment
Bacteriophage experiment. Proteins and DNA were tagged with a radioisotope and tracked as a virus attacked a bacteria. DNA entered cell
Chargaff Rule
A-T
Purine
A and G
Pyrimidine
T and C
Sugar Phosphate Backbone
a chain of alternating sugar and phosphate groups that forms the structural framework of DNA and RNA
Double Helix
twisting shape of DNA
Hydrogen Bond
bond due to polar charges
DNA Replication
process of copying DNA during S phase for Mitosis and Meiosis
Semiconservative Replication
the process by which DNA is copied to create two new DNA strands
Leading Strand
a strand of new DNA that is synthesized continuously and is complementary to the parental DNA strand
Lagging Strand
The lagging strand is synthesized in small fragments called Okazaki fragments.The lagging strand is synthesized discontinuously because the two strands of DNA run in opposite directions.
Topoisomerase
Flattens out DNA for replication
Helicase
“unzips” DNA by breaking hydrogen bonds between nitrogenous bases
SSB
keeps DNA separated after helicase separates the bases
RNA Primase
creates RNA primer so DNA polymerase III can attach
DNA Polymerase III
creates the complimentary strand of DNA to the original template strand
DNA Polymerase I
replaces the RNA primers with DNA
Ligase
connects the phosphate-sugar backbone at the end of replication
Primer
complementary section of RNA that provides an attachment point for DNA polymerase III
Okazaki Fragment
sections of complementary DNA created for the lagging strand during DNA replication
Protein Synthesis
creation of proteins
Transcription
complementary strand of mRNA created off of a template DNA strand for a gene