unknown test Flashcards by Joselyn Bello Ponce

what does Indole do? SIM

Some bacteria can hydrolyze a tryptophan, making indole as a byproduct.

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what are indole’s results

(+) red oily band
(-) green, beige, green

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Indole procedure

add 6 drops of Kovac’s reagent

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What does methyl red do?

Some bacteria can ferment glucose, making mixed acids as the byproduct.

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What are methyl red procedure

inoculate in MR/VP, add 3 drops of methyl red

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methyl red results

(+) instant pale-ish pink red
(-) no color change

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what does VP do?

some bacteria can ferment glucose, making 2,3 butanediol as byproduct.

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What are VP procedure

inoculate in MR?VP then add 8 drops of Barritt’s A and B reagent each.

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what are the results of VP

(+) red band after 25 min
(-) no color change

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what does citrate do?

some bacteria only use citrate as their sole carbon source, creating alkaline(basic) conditions as a byproduct.

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What is the citrate procedure

streak isolation on agar

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what are the results for citrate

(+) color change from green to blue
(-) color stays green

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what does PR Glucose do?(similar process with the rest of the carbohydrate

some bacteria can ferment glucose with acid and gas, using a durham

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what is the PR Glucose procedure

inoculate

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what are the PR Glucose results?

(+) bubbles and yellow
(-) no bubbles

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what does Urea Hydrolysis do?

if bacteria can hydrolyze urea where ammonia is produced as a byproduct

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What is Urea procedure

inoculate in urea broth

What are Urea results

(+) bright pink
(-) no bright pink

what does SIM-Sulfur do?

if bacteria can reduce sulfur to hydrogen sulfide Hs2 and forms ferrous salts to amke ferric sulfide

what does SIM- Motility do?

if bacteria can swim

what is the procedure for SIM-Sulfur and motility

inoculate by stabbing into sim agar, let sit for 24hrs then add 6 drops of kovac reagents

What is the results of SIM-Sulfur

insoluble black precipitate

What is the result of SIM- Motility

visible turbidity that extend outwards from the stab line

what does Nitrate reduction do?

Shows if bacteria can partially or fully reduce nitrates to nitrogen gas

what are Nitrate reduction prodcedures?

inoculate in broth and then add 5 drops of Nitrate reagent A IF no bubbles are present

what are Nitrate reduction results?

partial (+) tube turns red full reudction(+) bubbles present with no reagents (-) no gas bubbles, no color change afer adding reagents

What does Blood agar do?

asking if bacteria can hemolysis partially or fully destroy red blood cells

What are the blood agar procedure?

inoculate only , ayay!

Blood agar results

alpha hemolysis (+)= partial clearing of the red color of red agar, sometimes a green color present beta hemolysis(+)= full destruction of red blood cells, complete clearing gamma hemolysis(-)= no clearing stays red

What does CNA agar do?

its selective for gram positive( kills gram negative), and differential for hemolysis

What is the CNA procedure

streak-inoculate

What are the CNA agar results

(+) = will have gram-positive (+) = alpha or beta hemolysis (-)= will have gram-negative so no growth (-)= gamma hemolysis

what does MacConkey agar

selective for gram-negative (kills gram-positive) and differential for lactose fermentation aka is it able to ferment?

what is the procedure for MacConkey agar

Streak-inoculate

What are the results for MacConkey

(+)= will be gram-negative (+)= colonies are red or pink (-)= will have gram-positive so no growth (-)= no color change

what are the results for catalase?

(+)= bubbles = has catalase (-)= no bubbles

what does Catalase do?

if bacteria have the enzyme catalase, they can neutralize hydrogen peroxide. Which is necessary for aerobic metabolism aka cellular respiration

what is the procedure for catalase?

On a slide, place bacteria on one side and add 2-3 drops of hydrogen peroxide

What does Oxidase do?

tells us if bacteria have cytochrome C oxidase enzyme, which is used to transfer electrons from Cytochrome C in the ETC to oxygen. This is important for aerobic respiration

what is the procedure for oxidase

place bacteria on one side of the strip and the other side place P. aeruginosa. Add 2-3 drops of water on each side.

what is the result of oxidase

(+)= purple within 30 seconds (-)= no purple at all or in 30 seconds