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Vibrio Flashcards

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1
Q

species has been associated with large epidemics and bandencs

A

Vibrio

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2
Q

infections may play a role in Guillain-Barre (gbs) which occurs when the immune system harms the body’s nerves, leading to paralysis

A

Campylobacter

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3
Q

can cause ulcers and is linked to
gastric carcinoma

A

Helicobacter pylori

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4
Q

is commonly found in a wide variety of aquatic
environments, including fresh water, brackish or estuarine water, and marine or salt water

A

Vibrio

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5
Q

Most commonly isolated species in vibrionaceae

A
  1. Vibrio cholerea
  2. Vibrio parahaemolyticus
  3. Vibrio vulnificus
  4. Vibrio alginolyticus
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6
Q

Vibrio: MICROSCOPIC MORPHOLOGY

A

Curved (comma)
Asporogenous (nonspore)
In broth - possess polar, sheathed flagella
In solid media - peritrichous, unsheathed flagella

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7
Q

Vibrio clinical manifestation

A
  1. consumption of raw seafood
  2. immigration or foreign travel
  3. Gastroenteritis with cholera-ke or rice-water
    Stool
  4. AccIdental trauma incurred during contact associated water
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8
Q

Does not grow on MAC and glucose fermenter

A

Vibrio

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9
Q

Routine media used in vibrio

A

Thiosulfate-citrate-bile salts-sucrose agar (TCBS)

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10
Q

Vibrio general characteristics

A
  • Observed as a mucoid “stringing”
  • halophilic or “salt-loving” (except v. Cholorea and v. Mimicus)
  • Require the addition of sodium
  • Glucose fermenter
  • Does not grow mac
  • facultative anaerobe
  • catalase negative (v. Metschnikovii)
  • oxidase positive (v. Metschnikovii)
  • reduce nitrate to nitrite
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11
Q

Serotype of india

A

Ogawa

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12
Q

Serotype of Philippine

A

Inaba

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13
Q

Serotype of japan

A

Hikojima

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14
Q

causative agent of cholera

A

Vibrio cholerea O1 (asiatic cholera or epidemic cholera

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15
Q

is a disease of major public health significance for centuries.

A

Cholera

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16
Q

in what place do cholera prevalent in the

A

Bengal region of india and bangladesh

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17
Q

Spread through contaminated water

A

Cholera

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18
Q

The disease manifests in acute cases as a severe gastroenteritis accompanied by vomiting and followed by diarrhea

A

Cholera

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19
Q

Rice watery stool contain

A

Mucus flecks

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20
Q

cholera responsible for

A

cholera toxin or choleragen (powerful enterotoxin)

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21
Q

Treatment and management for cholera

A

Intravenous and oral fluid

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22
Q

Cholera Can be shorten by antibiotics such as

A

Azithromycin and ciprofloxacin

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23
Q

Two types of Cholera:

A
  1. Classical type of cholera
  2. El tor type of cholera
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24
Q

Commonlv known as Summer Diarrhea in Japan

A

Vibrio parahaemolyticus

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25
Second most common Vibrio species implicated in gastroenteritis
Vibrio parahaemolyticus
26
What type of vibrio is self-limited disease
Vibrio parahaemolyticus
27
Vibrio parahaemolyticus symptoms begin hours after ingestion of contaminated seafooc
24-48 hours
28
Produces a heat-stable hemolysin that is able to lyse human erythrocytes in a special, high salt mannitol medium
Kanagawa-toxin positive
29
What meduim is used in kanagawa-toxin positive
Wagatsuma agar
30
Least pathogenic; most frequently isolated
Vibrio alginolyticus
31
Two categories of infection: primary septicemia and wound infections
Vibrio vulnificus
32
Transport media for vibrio spp.
Carry-blair media
33
Recommended selective media for vibrio
Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS)
34
Difterentiates sucrose-termenting yellow colonies) species from nonsucrose-termenting (green colonies) species
Thiosulfate citrate bile salt-sucrose agar (TCBS)
35
Enrichment procedure for vibrio
alkaline peptone water with 1% sodium chloride can be inoculated at least 20 ml and incubated for 5 to 8 hours at 35° C
36
What non-lf colonies in vibrio
Macconkey agar
37
What hemolysis Is the SBA USED IN VIBRIO
A and B hemolysis
38
agar can give false-positive oxidase reactions in vibrio
Mac and cefsulodin irgasan novobiocin (cin)
39
colonies - medium to large colonies that appear smoon, opaque, and Inaescent witn a greenisn hue in vibrio
Sba and cap
40
Non-sucrose Sucro serermenter Fermenter
V. mimIcus, V. parahaemolyticus, V. damsela and most V. vulnificus strain Some V. Vulnificus strain
41
Sucrose fermenter
- V cholerae - V. alginolyticus, - V. fluvialis. - V. Furnissii, V. cincinnatiensis - V. metschnikovii
42
Prefers aquatic habitats; usually freshwater, estuarine and marine environments
Aeromonas
43
Aeromonas major clinical species
A. hydrophila A. Sobria A. caviae - most commonly isolated specie
44
Frequently isolated from animal meat products
Aeromonas
45
Aeromonas: GENERAL CHARACTERISTICS
Ubiquitous Oxidase + ) positive Glucose fermenter Gram-neaative rods Motile with single tuft of flagella (Lopotrichous' widelv distributed in tresnwater. estuarine, and marine environments worldwide
46
Aeromonas: CLINICAL MANIFESTATION
Enteric pathogens (different from Salmonella. Shiaella) cause diarrheal disease Diarrhea diarrheal infection is usually self-limiting Extraintestinal infections - septicemia and wound infections Most common presentation of wound infection cellulitis
47
most frequently associates with gastrointestinal infections
A. Caviea
48
was formerly classified with the Vibrios because of its Oxidase +) and microscopic moronooav.
Campylobacter
49
It colonizes the stomacn for a long time, causing a low-grade inflammatory process
Peptic ulcer (chronic superficial gastritis)
50
Most clinically relevant species: campylobacter
C. jejuni subsp. jejuni C. Coli C. Fetus
51
Mode of transmission of campylobacter
Direct contact Person to person
52
an autoimmune disorder characterized by acute paralysis caused by damage to the beronerd nervous systen
Guillain barre syndrome (GBs)
53
most common cause of bacterial gastroenteritis worldwide
C. jejuni
54
the causative agent of bacteremia
C. Fetus
55
major cause of type B gastritis or peptic ulcer
Helicobacter pylori
56
Important species of helicobacter
Helicobacter pylori
57
Delay in processing of stool specimen transport Meduim
Carry-blair media
58
Transport medium for Helicobacter pylori
Stuart medium
59
Specimen for Campylobacter causing gastroenteritis (C. jejuni:
Stool Rectal swab (less preferred)
60
Specimen of choice of C. fetus
Blood with incubation 35 - 37 degree
61
Specimen for Helicobacter
Gastric biopsy material
62
Commonly used selective media for C. jejuni:
CAMPY-Bap
63
Other media used: campylobacter
Butzler medium Skirrow medium
64
commonly usea media тог н. рут0r
CAP Brucella agar with 5% horse red blood cells (non selective)
65
Selective meduim for helicobacter
Skirrow’s agar
66
Incubation temperature: Campylobacter jejuni
42 degree
67
Incubation emperature: Helicobacter pylori
37 degree
68
Requires a noncapnophilic and capnophilic environment campylobacter
5 % O2 10% CO2
69
Requires a noncapnophilic and capnophilic environment helicobacter
5-10% O2 5-12% CO2
70
May appear as long spirals or 'S' or seagull-wing shapes
Campylobacter SPP.
71
Stain poorly on gram-stained smears
Campylobacter spp.
72
recommended as a counter stain of campylobacter
Carbol fuchsin
73
"Darting" motility (corkscrew movement)
Campylobacter
74
Curved or U-shaned
Helicobacter spp.
75
Motile with multiple flagella at one pole
Helicobacter
76
What species Is morphology smoot, convex, translucent colonies
C. Fetus
77
What species is morphology moist, runny looking, and spreading and Some are round and raised and others may be flat also non hemolytic
C. Jenuni and other enteric Campylobacter
78
2 oxidase positive
H. Pylori and C. Jejuni
79
What test is used to Differentiate h. Pylori And c. Jejuni
Urease test
80
Urease test positive and negative
Positive - h. Pylori Negative - c. Jejuni
81
Hippurate hydrolysis test positive
C. Jejuni

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