Week 10: Using Growth Patterns for Diagnosis and Treatment (Part 1)

Question 1

chicken

Answer 1

little

Question 2

name the types of media used in the lab

Answer 2

selective, differential, both

Question 3

define selective media

Answer 3

contains ingredients that inhibit the growth of what isn’t of interest

Question 4

define differential media

Answer 4

contains ingredients that cause colonies of the desired organism to appear different from many other species

Question 5

name five types of media we studied in lab

Answer 5

Bruce McConkey EMbaSSy

blood
macconkey
EMB
MSA
TSY

Question 6

selective or differential or both: blood agar

Answer 6

only differential

Question 7

selective or differential or both: MacConkey

Answer 7

both

Question 8

selective or differential or both: MSA

Answer 8

both

Question 9

selective or differential or both: TSY

Answer 9

neither

Question 10

name a differential media

Answer 10

blood

Question 11

name a selective media

Answer 11

antibiotic, PEA

Question 12

name selective and differential media

Answer 12

MSA, MacConkey

Question 13

when is blood agar used?

Answer 13

extensively used in clinical labs

Question 14

which media is extensively used in clinical labs?

Answer 14

blood agar

Question 15

what does blood agar support?

Answer 15

a wide variety of medically important bacteria

Question 16

supports a wide variety of medically important bacteria

Answer 16

blood agar

Question 17

colonies that produce proteins called hemolysins are surrounded by a zone of clearing

Answer 17

blood agar

Question 18

types of blood agar

Answer 18

alpha, beta, gamma hemolysis

Question 19

what does MacConkey media select for?

Answer 19

gram negative bacteria

Question 20

selects for gram negative bacteria

Answer 20

MacConkey media

Question 21

MacConkey is differential for what?

Answer 21

lactose fermenter

Question 22

differential for lactose fermenters

Answer 22

MacConkey

Question 23

how do lactose fermenters appear in macconkey agar?

Answer 23

pink

Question 24

pink on macconkey agar

Answer 24

lactose fermenters

Question 25

non fermenters in macconkey agar appear?

Answer 25

colorless

Question 26

colorless on macconkey agar

Answer 26

non fermenters

Question 27

what is macconkey agar designed to do? i.e. where do the bacteria come from that macconkey agar targets?

Answer 27

isolate bacteria that typically reside in the intestine

Question 28

what does macconkey agar contain?

Answer 28

bile salts, lactose, ph indicator

Question 29

bile salts are found in:

Answer 29

macconkey agar

Question 30

lactose is found in:

Answer 30

macconkey agar

Question 31

ph indicator is found in:

Answer 31

macconkey agar

Question 32

what is MSA designed to do?

Answer 32

isolate Staphylococcus species

Question 33

designed to isolate Staphylococcus species

Answer 33

MSA

Question 34

designed to isolate bacteria that typically reside in the intestine

Answer 34

macconkey

Question 35

what does the high salt concentration do in MSA?

Answer 35

inhibits most other organisms

Question 36

what is MSA checking for?

Answer 36

to see if colonies ferment the carbohydrate mannitol

Question 37

what does MSA do to be selective?

Answer 37

high salt concentration inhibits most other organisms

Question 38

how is MSA differential?

Answer 38

tests for mannitol fermenters by color change

Question 39

what color is MSA?

Answer 39

reddish pink

Question 40

reddish pink in MSA

Answer 40

color of medium

Question 41

yellow MSA medium

Answer 41

colonies that ferment mannitol

Question 42

what happens in MSA when colonies ferment mannitol?

Answer 42

the medium turns yellow

Question 43

which medium has more nutrients than minimal medium

Answer 43

TSY