Week 11: Biochemical tests and case study of impetigo caused by MRSA part 2

Question 1

what’s a more time-consuming and expensive strategy than metabolic and morphological analyses for species level identification

Answer 1

rDNA sequencing

Question 2

what were all these tests for?

Answer 2

identifying Gram-negative enteric bacteria

Question 3

what does VP test look for?

Answer 3

most VIPs are beautiful

if bacteria can utilize the butylene glycol pathway to produce acetoin or acetylmethylcarbinol

Question 4

what does indole test look for?

Answer 4

if the bacteria can degrade amino acid tryptophan

Question 5

what does citrate test look for?

Answer 5

C’s. Citrate and Carbon.

if bacteria can use citrate as carbon source

Question 6

what does urease test look for

Answer 6

if bacteria can hydrolyze urea to produce ammonia and CO2 using urease

Question 7

what does TSI test look for?

Answer 7

if bacterium 1. ferments glucose, 2. ferments lactose, 3. produces H2S or CO2

Question 8

what does the catalase test look for?

Answer 8

presence of catalase

Question 9

what does coagulase test look for?

Answer 9

coagulation due to enzyme secreted by Staph. aureus