Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

microbio lab > Week 2: Microscopy > Flashcards

Week 2: Microscopy Flashcards

1
Q

what are the three principles of microscopy?

A

magnification
resolution
contrast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what magnification should be used to focus my sample first? then what?

A

10X, then move to higher magnification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what height should i start at with the 10X lens?

A

maximum height, then move the stage down until it comes into focus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

100X always uses…

A

oil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

oil is always used with…

A

100X

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what magnification should i use on a unstained human red blood cell?

A

10X

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what contrast should i use on an unstained human red blood cell?

A

BF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what resolution should i use on a unstained human red blood cell?

A

air

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what magnification should i use on a capsule stained bacteria cell?

A

100X

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what contrast should i use on a capsule stained bacteria cell?

A

DF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what resolution should i use on a capsule stained bacteria cell?

A

oil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what magnification should i use for eukaryotic cells?

A

40X

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what magnification should i use for bacterial cells?

A

100X

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

when to use bright field?

A

live, no stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

when to use dark field?

A

capsule

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

when to use phase contrast?

A

unstained/colorless cells because staining provides a contrast

17
Q

what resolution with 100X?

A

oil

18
Q

what resolution with 10X?

A

air

19
Q

what resolution with 40X?

A

air

20
Q

when should i avoid bright field?

A

when viewing unstained wet mounts

21
Q

how do organisms appear in dark field?

A

bright and glowing against a dark background

22
Q

what magnification can and can’t we use with dark field?

A

CAN: 10X and 40X
CAN’T: 100X

23
Q

what is the best contrast setting for unstained live organisms?

A

phase contrast

24
Q

phase contrast is the best setting for…

A

unstained live organisms

25
Q

match phase ring to objective: PH1

A

10X

26
Q

match phase ring to objective: PH2

A

r40X

27
Q

match phase ring to objective: PH3

A

100X

28
Q

match objective to phase ring: 100X

A

PH3

29
Q

match objective to phase ring: 40X

A

PH2

30
Q

match objective to phase ring: 10X

A

PH1

31
Q

what contrast to use for 100X?

A

BF/phase contrast for 100X, not DF

32
Q

how to use oil immersion lens?

A
  1. 100X needs oil
  2. focus sample in 40X
  3. go back to 10X and put oil onto the slide.
  4. move 100X into place

microbio lab (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions