Western Blotting, Southern Blotting, Northern Blotting, and ELIZA

Question 1

What is Western blotting?

Answer 1

a technique that combines protein electrophoresis with antibody binding to identify target proteins with high specificity and sensitivity

Question 2

What does Western Blotting do?

Answer 2

separate proteins by size using gel electrophoresis, transfer to membrane, and detecting specific proteins with antibodies

Question 3

What technique is similar to Western blotting?

Answer 3

ELIZA

Question 4

What is the abbreviation ELIZA stand for?

Answer 4

Enzyme-linked immunoassay

Question 5

What is the abbreviation for Enzyme-linked immunoassay?

Answer 5

ELIZA

Question 6

How does ELIZA differ from Western blotting?

Answer 6

primarily differs because of its application and methodology

Question 7

What does ELIZA do?

Answer 7

uses antibodies to detect and quantify proteins directly in a liquid sample
- protein of interest binds to a solid surface and detection achieved with enzyme-linked antibody that produces a measurable signal (color change)

Question 8

What is ELIZA suitable for?

Answer 8

High throughput and quantitative analysis

Question 9

What is Southern Blotting for?

Answer 9

Detects specific DNA sequence within a complex DNA sample

Question 10

What is Northern blotting for?

Answer 10

Detects specific RNA sequences within a complex RNA sample