1-3 PL Quiz Flashcards Preview

Microbiology Lab > 1-3 PL Quiz > Flashcards

Flashcards in 1-3 PL Quiz Deck (18):
1

a medium that contains living microbes

culture

2

if a culture contains a single species

pure culture

3

what does aseptically mean

without contamination of yourself, others, the environment, the source culture, or the medium being inoculated

4

how must you transfer microbes from their pure culture to a sterile medium

aseptically

5

what is an LAI

laboratory acquired infection

6

what is a procedure's most important operational risk factor

potential to release microorganisms into the air as aerosols and droplets

7

minimize _________________________

the potential of contamination

8

be _________

organized

9

place all ________________________

media test tubes in a test tube rack when not in use whether they are sterile or not

10

take ______________

your time

11

never ______________

hold a tube culture by its cap

12

hold the __________________________________

inoculating loop or needle like a pencil in your dominant hand and relax

13

adjust _____________________________

your bunsen burner so its flame has an inner and outer cone

14

what are used to grow microbes when fresh cultures or large number of cells are required

broths

15

these are generally used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks

agar slants

16

these are typically used for obtaining isolation of species, differential testing, and quantifying bacterial densities

plated media

17

the instruments used for transfers are

inoculating loops and inoculating needles

18

what is the primary concern with BSL-2 organisms

contamination or infection due to inhalation of aerosols