3-1 PL Quiz Flashcards Preview

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Flashcards in 3-1 PL Quiz Deck (23):
1

produces an image made from light that is transmitted through a specimen

bright field microscopy

2

what are most biological specimens

transparent

3

how is the transparency of the specimen improved

application of stains to the specimen

4

what is the price of improved contrast of specimen

staining process kills cells

5

how does image formation begin

with light coming from an internal or external light source

6

concentrates the light and makes illumination of the specimen more uniform

condenser lens

7

another name for bending of light

refraction

8

when is the real image produced

during the refraction of light as it passes though the objective lens from the specimen

9

what appears below or within the microscope

virtual image

10

how is the total magnification of the specimen calculated

magnification by objective lens x magnification of ocular lens

11

what is the practical limit to magnification with a light microscope

1300x

12

what is clarity of an image called

resolution

13

another name for limit of resolution

resolving power

14

this is an actual measurement of how far apart two points must be for the microscope to view them as being separate

limit of resolution

15

resolution _______ as the resolution is made ______

improves;smaller

16

the best limit of resolution achieved by a light microscope is

.2 um

17

for a specific microscope the actual limit of resolution can be calculated by using

wavelength/ NA condenser + NA objective

18

the measure of a lens's ability to capture light coming from the specimen and use it to make the image

numerical aperture

19

using what increases the numerical aperture and makes its limit of resolution smaller

immersion oil

20

a special condenser is used so only the light reflected off the specimen enters the objective

dark field microscopy

21

uses special optical components to exploit subtle differences in the refractive indices of water to produce contrast

phase contrast microscopy

22

light waves that are out of place will produce what

a degree of cancellation or darkness

23

uses a fluorescent dye that emits fluorescence when illuminated with ultraviolet radiation

fluorescence microscopy