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Microbiology Lab > Exam 2 > Flashcards

Exam 2 Flashcards

1
Q

what does the combination medium of MR and VP tests contain

A

peptone, glucose, and phosphate buffer

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2
Q

what is the MR test designed to detect

A

organisms capable of performing mixed acid fermentation

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3
Q

acids produced my those capable of performing mixed acid fermentation tend to be what

A

stable

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4
Q

what does MR stand for

A

Methyl Red

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5
Q

what is mixed acid fermentation verified by

A

the addition of methyl red indicator dye following incubation

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6
Q

when is methyl red the color red

A

at a pH of 4.4

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7
Q

when is methyl red the color yellow

A

at a pH of 6.2

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8
Q

when is methyl red the color orange

A

with a pH in between 4.4 and 6.2

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9
Q

what is a positive, negative, and inconclusive result of the MR test

A

positive: red
negative: yellow
negative/inconclusive: orange

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10
Q

what is the VP test designed for

A

organisms that are able to ferment glucose but quickly convert their acid products to acetoin and 2,3 butanediol

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11
Q

what is the broad process of VP test

A

acetoin-diacetyl + guanidine- red color

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12
Q

what is a positive and negative result of the VP test

A

positive: red
negative: no color change

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13
Q

what does VP test stand for

A

Voges-Proskauer test

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14
Q

what family of bacteria are SIM used for and what type of rod are they

A

Enterobacteriaceae

gram negative rods

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15
Q

what three activities are SIM medium used for

A

sulfur reduction, indole production from tryptophan, and motility

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16
Q

what are the two ways that sulfur can be reduced

A

with the enzyme cystein desulfurase and thiosulfate reductase

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17
Q

what do both enzymes of sulfur reduction produce

A

H2S gas

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18
Q

when either sulfur reaction occurs in SIM medium, what occurs

A

FeS forms(black precipitate)

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19
Q

what is a positive and negative result of sulfur reduction

A

positive: blackening of medium(sulfur reaction)
negative: no blackening(no sulfur reaction)

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20
Q

what is indole production possible by

A

the presence of tryptophan

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21
Q

what can tryptophan hydrolysis be detected by the addition of and what does it contain

A

Kovac’s reagent which contains DMABA

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22
Q

what is a positive and negative result of indole production

A

positive: red(presence of tryptophanase)
negative: no red color (doesn’t make tryptophase)

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23
Q

what is motility made possible by

A

the reduced agar concentration and the method incoulation

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24
Q

what is an indication of motility

A

growth that radiates in all direction and appears slightly fuzzy is an indication of motility

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25
what does IMViC stand for
Indole, methyl red, voges-proskauer, and citrate
26
what is IMViC used for
to distinguish between members of the family Enterobacteriaceae
27
what is TSIA designed to differentiate between
glucose fermentation, lactose fermentation, sucrose fermentation, and sulfur reduction
28
what is TSIA composed of
3 carbohydrates, animal proteins, ferrus sulfate and sodium thiosulfate
29
what is the pH indicator of TSIA
phenol red
30
what is the hydrogen sulfide indicator
iron
31
what can show up as a false positive because changes back to basic after all glucose is used up
reversion
32
KIA is identical to TSIA in form and function except what
KIA does not include sucrose
33
yellow slant, yellow butt KIA
glucose and lactose fermentation with acid accumulation in slant and butt
34
yellow slant, yellow butt TSIA
glucose and lactose and or sucrose fermentation with acid accumulation in slant and butt
35
red slant and yellow butt (TSIA)
glucose fermentation with acid production, proteins catabolized aerobically with alkaline products
36
red slant and red butt (TSIA)
no fermentation, peptone catabolized aerobically and anaerobically with alkaline products, not from enterobacteriaceae
37
red slant, no change in butt (TSIA)
no fermentation, peptone catabolized aerobically with alkaline products, not from enterobacteriaceae
38
no change in slant and no change in butt (TSIA)
organism is growing slowly or not at all, not from enterobacteriaceae
39
black precipitate in agar (TSIA)
sulfur reduction(acid condition), from fermentation of glucose or lactose, exists in the butt even if the yellow color is obscured by the black precipitate
40
crack in a lifting of agar (TSIA)
gas production
41
what does the citrate utilization test tell us about
organism's ability to use citrate as their sole carbon source and perform citrate fermentation
42
what is a defined medium and give an example of it
amount and source a of all ingredients are carfeully controlled and simmons citrate agar is an example
43
bacteria that does not possess citrate permase will not grow on what medium
ammonium as the sole nitrogen source
44
this is the enzyme citrate pos bacteria use to hydrolyze citrate into oxalocetate and acetate(oxalocetate is then converted to pyruvate)
citrate lyase
45
what is a positive result of the citrate utilization test
blue and no color change but GROWTH (citrate is utilized)
46
what is a negative result of the citrate utilization test
no color change and no growth (citrate is not utilized)
47
metabolic process by which an organic molecule acts as an electron donor and one ore more of its organic products as the FEA
carbohydrate fermentation
48
what is carbohydrate fermentation used to include
hydrolysis of disoccharrides prior to fermentation
49
organism that splits the disaccharide lactose and ferments the monosccharides
lactose fermenter
50
phenol red broth and MR and VP broth
differential fermentation media
51
this is a differential test medium prepared as a base to which a carbohydrate is added
phenol red broth
52
what does phenol red broth include
peptone and phenol red
53
what it the pH indicator of phenol red broth
phenol red
54
when is phenol red the color yellow
below pH of 6.8
55
when is phenol red the color pink
above pH of 7.4
56
when is phenol red the color red
in between pH of 6.8 and 7.4
57
this is added to each tube as an indicator of gas production from fermentation, indicated by a buubble
inverted Durham tube
58
yellow broth and bubble in tube
fermentation with acid and gas end products
59
yellow broth and no bubble in tube
fermentation with acid end products, no gas produced
60
red broth and no bubble in tube
no fermentation
61
pink broth and no bubble in tube
degradation of peptone and alkaline end products

Microbiology Lab (25 decks)

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