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Microbiology Lab > Exam 3 Part 2 > Flashcards

Exam 3 Part 2 Flashcards

(61 cards)

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1
Q

reactions that use water to split complex molecules

A

hydrolysis

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2
Q

these catalyze reactions inside cells

A

intracellular enzymes

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3
Q

these catalyze reactions outside cell

A

extracellular enzymes or exoenzymes

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4
Q

what identify extracellular enzymes

A

starch agar, DNase agar, tributyrin agar, milk agar

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5
Q

exoenzyme that liquifies solid medium

A

gelatinase

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6
Q

polysaccharide made up of < D glucose subunits

A

starch

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7
Q

starch is either

A

linear (amylose) or branched (amylopectin)

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8
Q

is starch large

A

yes too big to pass through membrane so must be split into individual glucose molecules

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9
Q

what does starch agar contain

A

beef extract, soluble starch, and agar

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10
Q

what is used to detect presence or absence of starch in vicinity around the bacterial growth

A

reagent iodine

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11
Q

what does a clearing around growth on starch agar mean

A

< amylase and or oligo-1, 6 glucosidase is present so positive result

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12
Q

what does no clearing around growth on starch agar mean

A

< amylase and or oligo-1 6 glucosidase is NOT present so negative result

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13
Q

what does Columbia CNA allow growth of and what does it inhibit growth of

A

allows growth of gram positive and inhibits growth of gram negative

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14
Q

what is Columbia CNA composed of

A

casein, digest of animal tissue, beef extract, yeast extract, corn starch, and sheep blood

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15
Q

what do the yeast and blood of columbia CNA do

A

yeast provides vitamins and blood supplies heme

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16
Q

what is selective against gram negative in Columbia CNA

A

antibiotic colistin and nalidixic acid

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17
Q

what does sheep blood make possible

A

differentiation of gram positive organisms based on hemolytic reaction

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18
Q

what is Columbia CNA agar used to isolate

A

Staphylococci, streptococci, and enterococci

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19
Q

what does poor or no growth on columbia CNA mean

A

inhibited by colistin and nalidixic acid so gram negative

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20
Q

what does good growth with clearing on columbia CNA mean

A

not inhibited by colisitn and nalidixic acid and completely hemolyzes RBCs so B hemolytic

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21
Q

what does good growth with greening on columbia CNA mean

A

not inhibited by colisitn and nalidixic acid and partially hemolyzes RBCs so < hemolytic

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22
Q

what does good growth with no color change on columbia CNA mean

A

not inhibited by colisitn and nalidixic and does NOT hemolyze RBCs so y hemolytic

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23
Q

what does mannitol salt agar contain

A

carbohydrate mannitol, 7.5 % sodium chloride, and pH indicator phenol red

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24
Q

when is phenol red different colors

A

yellow below 6.8, red 7.4-8.4 and pink above 8.4

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25
what does the mannitol in salt agar do
provides the substrate for fermentation and makes the medium differential
26
what does the sodium chloride in mannitol salt agar do
makes medium selective as it kills most
27
what bacteria thrive on mannitol salt agar
Staphylococci
28
this indicates if fermentation with acid end products occured
phenol red
29
mannitol salt agar is used for
isolation and differentiation of staphylococcus aureus from other staphylococcus species
30
poor or no growth on mannitol salt agar
inhibited by NaCl so not staphylococcus
31
good growth on mannitol salt agar
not inhibited by NaCl so staphylococcus
32
yellow or halo on mannitol salt agar
produces acid from mannitol fermentation so pathogenic staphylococcus aureus
33
red growth and no halo on mannitol salt agar
does not ferment mannitol so staphylococcus but NOT aureus
34
what is another name for Eosin Mthyene Blue
Levine
35
what does EMB contain
digest of gelatin, lactose, and dyes esoin y and methylene blue
36
what does digest of gelatin provide EMB
organic carbon and nitrogen
37
what are the purposes of eosin y and methylene blue
1) inhibit growth of most gram positive | 2) react with vigorous lactose fermenters whose acid end products turn growth purple/black
38
dark growth and green metallic sheen is typical of what on EMB
E. coli
39
what is EMB used for isolation of
fecal coliforms
40
poor or no growth on EMB
inhibited by dyes so gram positive
41
good growth on EMB
not inhibited by dyes so gram negative
42
pink/mucoid growth on EMB
ferments lactose with little acid production so possible coliform
43
dark growth on EMB
ferments lactose and or sucrose with acid production so probable coliform
44
colorless growth on EMB
does not ferment lactose or sucrose so noncoliform
45
what is blood agar useful for detecting
detecting hemolytic ability of gram positive cocci
46
this is produced by some gram positive cocci | exotoxins that destroy RBCs and hemoglobin
hemolysins
47
what does blood agar contain
5% blood in tryptic soy agar base
48
what is B hemolysis
complete destruction of RBCs and hemoglobin | results in clearing of medium around colonies
49
what is < hemolysis
partial destruction of RBCs and hemoglobin | produces greenish decolorization of agar around colonies
50
what is y hemolysis
nonhemolysis, appears as simple growth | no change to medium
51
hemolysins produced by steptococci
streptolysins
52
streptolysin O
oxygen liable (optimal is anaerobic conditions)
53
streptolysin S
oxygen stable(optimal under anaerobic conditions)
54
what technique is used for blood agar
streak and stab for isolation and encourage activity
55
what is the 21st test of api 20 E
oxidase test
56
what is the seven digit code interpreted on
analytic profile index
57
what is the 21st test of api 20 E
oxidase test
58
what is the seven digit code interpreted on
analytic profile index
59
what is api 20 E multi test system used for
clinically for rapid identification of Enterobacteriaceae and other gram negative rods
60
what is the purpose of the water in api 20 E
humidify the strip during inoculation
61
describe coliforms
``` rod shaped, gram negative non spore forming ferment lactose with production of acid/gas indicator of sanitary food quality example is E. coli ```

Microbiology Lab (25 decks)

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