biotechnology

Question 1

biotechnology

Answer 1

manipulation of living organisms using genetic engineering to make desired products

Question 2

recombinant dna technology

Answer 2

combining strands of dna by molecular cloning

Question 3

recombinant dna

Answer 3

creating new sequences

rdna is called chimera

Question 4

molecular cloning

Answer 4

fragment of dna from donor to plasmid that replicate in host

Question 5

plasmid

Answer 5

double stranded dna
circular
plasmid is also known as vectors

Question 6

steps of cloning

Answer 6

1selection of host organism and cloning vector
2prep of vector dna
3prep of dna to be cloned

Question 7

last 4 steps of cloning

Answer 7

4construct recombinant dna
5introduce recombinant dna into host organism
6selection of organism
7screening for clones

Question 8

cloning vector

Answer 8

a genome which accept the target dna and through replicate

Question 9

molecular cloning

Answer 9

• **carried in vitro
• **fragment from donor to vector
• **into plasmid
• ***replicates in host cell

Question 10

plasmids are

Answer 10

with double stranded dna
found inside bacterial species
also known as vectors

Question 11

types of vectors

Answer 11

plasmid 
bacteriophage
cosmid
YAC
BAC