Serological reaction Flashcards
Serolo reaction
Antigen antibody reaction
To determine presence of antigen or antibody
Types of serological
Agglutination
Precipitation
Immuno electrophoresis
Western blot
Agglutination
Antigen antibody crosslinks
Form complexes
Visible clumbs
Antibody is…… In agglutination
Divalent
Haemagglutination test
Virus clump rbc s
Inhibited by anti viral antibodies
Pasive haemagglutination
Coating carrier molecules. With antigens
Latex agglu
Haemagglutination
Precipitation reaction
Antigen and antibody mixed in proportion
Can see large macromolecular complexes
Called precipitation
Single radial immunodiffusion
Antibody in agar
Antugen in well
Antigen diffuse precipitation ring form
To measure Ig G Ig M complement compounds
Double immunodiffusion
Antigen and antibody in seperate wells
Allowed. To. React
Precipitation reaction when in optimum. Conc
Radioimmune assay
Measure Ig E
Complement fixation
Antigen antibody reaction activates complement Needs Patient serum antibody. Known antugen Complement Erythrocytes from sheep Haemolyting serum
Immunoflorescence testing
Uses flurescent antibody to visualise cell or cell aggregates
Immunoflorescent testing
Uses fluorescene isothiocyanate labelled immunoglobulin
Herpes rabies
Elisa
To detect unknown antigen or antibody
A positive result colour production
Sensitize plate
Wash
Add antibody..
Wash
Add ligand
Wash
Adf chromogen
Develop plate
Immunoelectophoresis
Serum protein migration combined with antibody precipitation
