Stem Cell Quiz Flashcards
Which of the following are common sites used by veterinarians to harvest adipose tissue for processing?
Select one or more:
a.
Spinal region
b.
Abdominal (omental) region
c.
Scapula region
d.
Abdominal (falciform) region
Scapula region
Abdominal (omental) region
Abdominal (falciform) region
Adipose-derived mesenchymal stem cells may have a primary mode of action in aiding repair in damaged tissues due to their differentiation potential.
Select one:
True
False
‘True’.
In the caprine osteoarthritis model, the stem cell treatment group observed what in comparison to the control group? (mark all that apply)
Select one or more:
a.
No significant differences were observed
b.
Greater biomechanical strength
c.
Reduced cartilage breakdown
d.
Greater thickness of joint cartilage
Greater thickness of joint cartilage
Reduced cartilage breakdown
Greater biomechanical strength
The Stromal Vascular Fraction (SVF) contains cultured MSCs for administration.
Select one:
True
False
‘False
What are the most common sources used for isolation of adult stem cells in veterinary medicine?
Select one or more:
a.
Embryo
b.
Bone marrow
c.
Adipose tissue
d.
Dental pulp
Bone marrow
Adipose tissue
Compared to bone marrow aspiration, when isolated from adipose tissue, culturing of stem cells to achieve a therapeutic dose is not necessary due to the high concentration of cells within the tissue.
Select one:
True
False
‘True’.
What frequency range was found to inhibit growth and proliferation of adipose-derived mesenchymal stem cells?
Select one:
a.
Red
b.
Green
c.
Blue
d.
Yellow
Blue
In-clinic tissue processing being introduced to veterinarians around the country had which of the following effects? (mark all that apply)
Select one or more:
a.
Lowered cost to client
b.
Eliminated transit time
c.
Added additional days to the process
d.
Raised cost to client
Eliminated transit time
Lowered cost to client
When administered intravenously, adipose-derived mesenchymal stem cells home to damaged tissue via chemotactic distress signals that are secreted by the injured tissue.
Select one:
True
False
True’.
Cellular respiration, or oxidative metabolism, is one of the primary means for cells to gain useful energy by converting biochemical energy, taken from nutrients, into ATP.
Select one:
True
False
‘True’.
The largest colony size of canine adipose-derived stem cells in vitro were treated with which of the following:
Select one:
a.
Neither PRP nor photobiostimulation
b.
Platelet –Rich Plasma (PRP)
c.
Photobiostimulation
d.
Both PRP and photobiostimulation
Both PRP and photobiostimulation
In a University project, an activated sample (Platelet Rich Plasma + Light) showed the greatest potential for which of the following? (mark all that apply)
Select one or more:
a. Gene Expression (secretion)
b.
MSC potential
c.
Cell Death
d.
Viability
Gene Expression (secretion)
MSC potential
Viability
Currently there are no studies demonstrating the benefits of Adipose Derived Stem Cells for therapeutic treatment of canine osteoarthritis.
Select one:
True
False
False’.
The vital growth factors released from the platelets in PRP are responsible for “activating” the stem cells resulting in increased:
Select one or more:
a.
Proliferation of cells
b.
Apoptosis
c.
Cyto-protective to the stem cell population
Proliferation of cells
Cyto-protective to the stem cell population
What is the fate of the adipose-derived stem cells once they are implanted within the body? (mark all that apply)
Select one or more:
a.
Secrete growth factors
b.
Attract growth of blood vessels
c.
Differentiate into component cell types
d.
Disperse into adjacent tissue
Disperse into adjacent tissue
Differentiate into component cell types
Secrete growth factors
Attract growth of blood vessels
