Flashcards in 1.1 + 1.2 esophagus etc; 1.3 fem reprod - 1st wk embryo Deck (78)
stains nuclei blue in H&E stain
basophilic stain in H&E stain
acidophilic stain in H&E stain
stains cytoplasm & connective tissue pink in H&E stain
PAS stain stands for
periodic acid-Schiff stain
PAS stains nuclei what color
stains glycoproteins pink
stains basement membranes pink
stains laminin pink
stains microvilli pink
stains cellular and connective tissue different colors
Azan stain or another "trichrome stain"
approximate light microscope resolution?
typical light microscope section thickness
lines blood vessels
outer layer of an organ consisting of epithelial layer (mesothelium) and connective tissue layer
outer layer of an organ consisting of connective tissue without epithelial layer
adventitia vs serosa
outer layer of organ -- adventitia just connective tissue vs serosa connective tissue and epithelial boundary
layer surrounding all orifices, wet
simple squamous epithelial layer covering all true serous membranes
smooth muscle layer nearest the lumen of the esophagus
muscle layer adjacent to the serosa of the esophagus
connective tissue subjacent to the mucosa
non-mucosal smooth muscle circling the esophagus
inner circular layer
non-mucosal smooth muscle outside the inner circular layer, running longitudinally along the esophagus
outer longitudinal layer
connective tissue layer within the mucosa connecting the mucosal epithelium and the muscularis mucosae
the muscular layers of the esophageal muscularis externa
inner circular layer; outer longitudinal layer
three layers of the esophageal mucosa
mucosal epithelium, lamina propria, muscularis mucosae
what is the name of the muscular layer of a tubular organ if the muscularis mucosae is absent?
what is the name of the muscular layer of a tubular organ if the muscularis mucosae is present?
man-made structure created in processing
is mitochondria eosinophilic or hematoxophilic?
why is it unusual to see the cell membrane by light microscopy?
thickness is small ~ 7.5 nanometers. LM resolution is ~ 1 micron
why do cells sometimes shrink away from each other in processing? e.g. smooth muscle cells separating into fascicles
dehydration by immersion in increasing concentrations of alcohol
name an anuclear cell
red blood cell
name 9 tissue layers of the esophagus
from lumen out
mucosa: -epithelium, -lamina propria, -muscularis mucosae
muscularis externa: -inner circular layer, connective tissue, -outer longitudinal layer
-adventitious/serous connective tissue
-mesothelium (serous epithelium)
name for the epithelial layer of the serosa
glycogen, glycoproteins, proteoglycans -- which 2 do PAS stain?
glycogen and glycoproteins, NOT proteoglycans
difference between glycoprotein and proteoglycan
glycoprotein = macromolecule containing glycogen and protein
proteoglycan = class of glycoprotein consisting of higher carb/protein ratios
what are two modern names for fallopian tube?
oviduct; uterine tube
two main functions of the ovary
2 hormones secreted by ovary
3 regions of of uterine tube
fimbrae - sweep in oocyte from peritoneal cavity
infundibulum - funnel
ampulla - tube & usual site of fertilization
3 layers of uterus
2 layers of endometrium
functionalis - nurtures zygote or sloughs
basalis - stem cell layer
layer of endometrium that nurtures zygote in pregnancy or sloughs off in menstrual cycle
stem cell layer of endometrium - not sloughed
3 phases of menstrual cycle
site of oocyte maturation
progression of ovarian follicular development to ovulation
-primordial follicle: oocyte surrounded by flat layer of follicular cells, developed before birth
-primary follicle: growth; follicular cells become granulosa cells; zona pellucida develops. this stage begins at puberty
-secondary follicle - fluid filled antrum develops within granulosa cells
-graafian follicle - mature follicle chosen for ovulation, ~day 14 of menstrual cycle
zona pellucida - what is it, when does it develop, when does it degenerate?
glycoprotein membrane surrounding the plasma membrane of an oocyte; develops in the primary follicle; degenerates for implantation
3 innermost layers of an ovulated secondary oocyte
granulosa cells (corona radiata)
fluid filled compartment that develops in the secondary follicle
what is the name of the mature follicle just before ovulation?
structure between uterus and vagina
male gamete is called:
female gamete is called:
contains enzymes to penetrate the corona radiata and zona pellucida of the oocyte
acrosome cap of the sperm
reaction when sperm reaches plasma membrane of oocyte that prevents multiple sperm from entering oocyte
zona reaction -- reaction of the zona pellucida
when sperm and oocye nuclei exist in plasma before fusing they are called:
fusion of sperm and oocyte results in a...
12 cell embryo stage is called...
embryo is called a ______ when entering uterus
morula - 12 cell stage
morula enters uterus ___ days post fertilization
map from fertilization to implantation
2 cell stage
4 cell stage
12 cell stage - morula - 3-4 days post fert
blastocyst - implantation - 6 days post fert
2 parts of blastocyst
embryoblast - inner cell mass - rise of tissues
trophoblast - outer cell mass - rise of placenta
degenerates to allow implantation
inner cell mass of blastocyst - gives rise to tissues of embryo
outer cell mass of blastocyst - gives rise to placenta
"trophe" = nutrition
how many days post fertilization does implantation occur?
how many days into the menstrual cycle does fertilization typically occur?
what phase of the menstrual cycle does fertilization typically occur?
tube of oviduct and typical site of fertilization
structure that sweeps in ovulated oocyte from peritoneal cavity
funnel between the fimbrae and ampulla of oviduct
oocyte surrounded by flat layer of follicular cells, developed before birth
growth; follicular cells become granulosa cells; zona pellucida develops. this stage begins at puberty
fluid filled antrum develops within granulosa cells