1.1 Microscopy

Question 1

What were the first type of microscope?

Answer 1

Light

Question 2

What are the two types of lense in a compound light microscope?

Answer 2

Objective and eyepiece

Question 3

What are the parts of a microscope?

Answer 3

Eyepiece lense
Turret
objective lense 
coarse focusing knob
fine focusing knob
specimen stage

Question 4

What types of mount are there?

Answer 4

Dry - cover slip placed over slice of specimen

Wet - specimen in liquid and cover slip placed on from angle

Question 5

Define Magnification

Answer 5

how many time larger the image is than the actual object

Question 6

Define Resolution

Answer 6

ability to see individual objects as separate objects

Question 7

What limits resolution? and how it can be resolved?

Answer 7

It is limited by diffraction of light

Use bean of electrons which have wavelength thousands time shorter than light

Question 8

Calculation for magnification

Answer 8

mag = size of image / actual size

Question 9

How man micrometers (um) are in a millimetre (mm)

Answer 9

1000

Question 10

how do you get from nanometers to micrometers

Answer 10

divide by 1000

300nm = 0.3 um

Question 11

What are the 3 measurements in microscopy?

Answer 11

Nanometres nm
Micrometers um
Millimetres mm

Question 12

What is the difference between magnification and resolution?

Answer 12

Mag is how much bigger image is then original image

Resolution is ability to distinguish between two points

Question 13

Whats resolution of light microscope

Answer 13

50-200nm

Question 14

What is resolution of transmission electron microscope

Answer 14

0.05-1.0nm

Question 15

What is benefit of using scanning electron microscope?

Answer 15

Produced 3D image with surface detail

Question 16

How does a laser scanning microscope work

Answer 16

laser beam scan specimen and pick up fluorescent and is focused through pinhole

Question 17

What kind of image does a laser scanning confocal produce

Answer 17

3D

Question 18

What are the two types of electron microscope

Answer 18

Transmission and scanning

Question 19

How does Transmission electron microscope work

Answer 19

Use electromagnets to focus electrons that gather at denser parts

Question 20

What is resolution of TEM

Answer 20

0.0002um

x100 000

Question 21

What does TEM stand for

Answer 21

Transmission Electron Microscope

Question 22

What does SEM stand for

Answer 22

Scanning Electron Microscope

Question 23

How does SEM work

Answer 23

Scan a beam of electrons off and knock off electrons and are gathered up by cathode

Question 24

What kind of image does SEM produce

Answer 24

3D

Question 25

What is resoloution and mag for SEM

Answer 25

0.002um x500 000

Question 26

What is resolution and mag of TEM compared to SEM

Answer 26

TEM x100 000 0.0002um SEM x500 000. 0.002um

Question 27

What is purpose of staining specimen

Answer 27

To make contrast like methylene blue

Question 28

What is used to create contrast in specimen for electron microscope

Answer 28

Dip specimen in heavy metal which cause electrons to scatter

Question 29

What is used to focus on magnifier

Answer 29

Fine adjustment knob