Flashcards in 1.1 Microscopy Deck (29):
What were the first type of microscope?
What are the two types of lense in a compound light microscope?
Objective and eyepiece
What are the parts of a microscope?
coarse focusing knob
fine focusing knob
What types of mount are there?
Dry - cover slip placed over slice of specimen
Wet - specimen in liquid and cover slip placed on from angle
how many time larger the image is than the actual object
ability to see individual objects as separate objects
What limits resolution? and how it can be resolved?
It is limited by diffraction of light
Use bean of electrons which have wavelength thousands time shorter than light
Calculation for magnification
mag = size of image / actual size
How man micrometers (um) are in a millimetre (mm)
how do you get from nanometers to micrometers
divide by 1000
300nm = 0.3 um
What are the 3 measurements in microscopy?
What is the difference between magnification and resolution?
Mag is how much bigger image is then original image
Resolution is ability to distinguish between two points
Whats resolution of light microscope
What is resolution of transmission electron microscope
What is benefit of using scanning electron microscope?
Produced 3D image with surface detail
How does a laser scanning microscope work
laser beam scan specimen and pick up fluorescent and is focused through pinhole
What kind of image does a laser scanning confocal produce
What are the two types of electron microscope
Transmission and scanning
How does Transmission electron microscope work
Use electromagnets to focus electrons that gather at denser parts
What is resolution of TEM
What does TEM stand for
Transmission Electron Microscope
What does SEM stand for
Scanning Electron Microscope
How does SEM work
Scan a beam of electrons off and knock off electrons and are gathered up by cathode
What kind of image does SEM produce
What is resoloution and mag for SEM
What is resolution and mag of TEM compared to SEM
x100 000 0.0002um
x500 000. 0.002um
What is purpose of staining specimen
To make contrast like methylene blue
What is used to create contrast in specimen for electron microscope
Dip specimen in heavy metal which cause electrons to scatter