1.1 Microscopy Flashcards Preview

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Flashcards in 1.1 Microscopy Deck (29):
1

What were the first type of microscope?

Light

2

What are the two types of lense in a compound light microscope?

Objective and eyepiece

3

What are the parts of a microscope?

Eyepiece lense
Turret
objective lense
coarse focusing knob
fine focusing knob
specimen stage

4

What types of mount are there?

Dry - cover slip placed over slice of specimen

Wet - specimen in liquid and cover slip placed on from angle

5

Define Magnification

how many time larger the image is than the actual object

6

Define Resolution

ability to see individual objects as separate objects

7

What limits resolution? and how it can be resolved?

It is limited by diffraction of light
Use bean of electrons which have wavelength thousands time shorter than light

8

Calculation for magnification

mag = size of image / actual size

9

How man micrometers (um) are in a millimetre (mm)

1000

10

how do you get from nanometers to micrometers

divide by 1000

300nm = 0.3 um

11

What are the 3 measurements in microscopy?

Nanometres nm
Micrometers um
Millimetres mm

12

What is the difference between magnification and resolution?

Mag is how much bigger image is then original image
Resolution is ability to distinguish between two points

13

Whats resolution of light microscope

50-200nm

14

What is resolution of transmission electron microscope

0.05-1.0nm

15

What is benefit of using scanning electron microscope?

Produced 3D image with surface detail

16

How does a laser scanning microscope work

laser beam scan specimen and pick up fluorescent and is focused through pinhole

17

What kind of image does a laser scanning confocal produce

3D

18

What are the two types of electron microscope

Transmission and scanning

19

How does Transmission electron microscope work

Use electromagnets to focus electrons that gather at denser parts

20

What is resolution of TEM

0.0002um

x100 000

21

What does TEM stand for

Transmission Electron Microscope

22

What does SEM stand for

Scanning Electron Microscope

23

How does SEM work

Scan a beam of electrons off and knock off electrons and are gathered up by cathode

24

What kind of image does SEM produce

3D

25

What is resoloution and mag for SEM

0.002um

x500 000

26

What is resolution and mag of TEM compared to SEM

TEM

x100 000 0.0002um

SEM

x500 000. 0.002um

27

What is purpose of staining specimen

To make contrast like methylene blue

28

What is used to create contrast in specimen for electron microscope

Dip specimen in heavy metal which cause electrons to scatter

29

What is used to focus on magnifier

Fine adjustment knob