Topic8 RNAi And siRNA

Question 1

What does RNAi stand for?

Answer 1

RNA interference

Question 2

What does siRNA stand for?

Answer 2

Small interfering RNA

Question 3

What is RNAi?
What does it do?

Answer 3

In eukaryotes and some prokaryotes
Translation of the mRNA produced from target genes can be inhibited by RNAi

Question 4

How is translation of mRNA inhibited by RNAi?

Answer 4

This happens when mRNA molecule that’s already been transcribed gets destroyed before it’s translated

This is done by siRNA (small interfering RNA)

Question 5

What does siRNA control?

Answer 5

siRNA control the translation control

Question 6

What is the first thing that occurs in siRNA?

Answer 6

Enzymes cuts mRNA into siRNA

siRNA is double stranded and one strand of siRNA then combines with an another enzyme

Question 7

How is siRNA formed?

Answer 7

siRNA is formed when an enzyme cuts mRNA into siRNA

So siRNA comes from mRNA

Question 8

What is the structure of siRNA?

What is the second step in how does siRNA occur?

Answer 8

Structure of siRNA is double stranded and one strand of siRNA combines with another enzyme

Forms siRNA-enzyme complex and bind to complementary base pairing to another mRNA molecule as its singles stranded.

Question 9

What is the structure of mRNA?

Answer 9

Short single stranded chain of polynucleotides

Question 10

What is the last thing that occurs in how does siRNA occur?

Answer 10

When siRNA enzyme complex bind to another mRNA molecule.

Enzyme will cut up the mRNA so cannot be translated