Investigating Enzymes Flashcards

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1
Q

effect of temperature on enzymes experiment

A
  • (any enzyme can be used to investigate the effect of temperature)
  • amylase digests starch - mix 10cm3 of 10% starch suspension and 5cm3 of 5% amylase in a boiling tube and heat in a water bath
  • every minute (from the point mixing occurs) take one drop of this mixture and add it to one drop of iodine solution in the spotting tile
  • when digestion is complete and no starch is present, iodine solution will stay orange (rather than turning blue/black
  • repeat this using water baths of different temperatures
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2
Q

effect of pH on enzymes?

A
  • (you can use any enzyme to investigate the effect of pH)
  • potato contains the enzyme catalase, which breaks down hydrogen peroxide, forming water and oxygen gas
  • add 10 potato disks (1m thick, cut with a cork borer) to 20cm3 catalase and measure the volume of gas produced in 5 minutes
  • the pH can be altered by adding different volumes of a weak alkali or a weak acid - use universal indicator paper to determine the pH of the solution each time
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