Investigating Enzymes Flashcards
1
Q
effect of temperature on enzymes experiment
A
- (any enzyme can be used to investigate the effect of temperature)
- amylase digests starch - mix 10cm3 of 10% starch suspension and 5cm3 of 5% amylase in a boiling tube and heat in a water bath
- every minute (from the point mixing occurs) take one drop of this mixture and add it to one drop of iodine solution in the spotting tile
- when digestion is complete and no starch is present, iodine solution will stay orange (rather than turning blue/black
- repeat this using water baths of different temperatures
2
Q
effect of pH on enzymes?
A
- (you can use any enzyme to investigate the effect of pH)
- potato contains the enzyme catalase, which breaks down hydrogen peroxide, forming water and oxygen gas
- add 10 potato disks (1m thick, cut with a cork borer) to 20cm3 catalase and measure the volume of gas produced in 5 minutes
- the pH can be altered by adding different volumes of a weak alkali or a weak acid - use universal indicator paper to determine the pH of the solution each time