exam 2 lecture 12

Question 1

T/F CPCRs are the most targeted human protein family for drug development

Answer 1

True

Question 2

GPCR signal termination is cause by

Answer 2

phosphorylation by G protein-coupled receptor kinases GRKs

Question 3

binding of beta arrestin in GPCRs causes what

Answer 3

desensitization

Question 4

recruitment to Clathrin coated pits in GPCR termination causes what

Answer 4

internalization

Question 5

G protein inactivation uses what to covert active form to inactive form

Answer 5

intrinsic GTPase activity

Question 6

G protein inactivation is facilitated by

Answer 6

regulators of G protein signaling proteins RGSs

Question 7

alpha i leads to

Answer 7

ion channels, inhibition cAMP, phospholipase, phosphodiesterases

Question 8

alpha s leads to

Answer 8

increases cAMP

Question 9

alpha q leads to

Answer 9

phospholipase C

Question 10

alpha 12 leads to

Answer 10

Rho GEFs activation

Question 11

G alpha has how many subtypes

Answer 11

20

Question 12

what is Gs

Answer 12

dopamine D1 beta adrenergic receptors

Question 13

what is Gi

Answer 13

dopamine D2 alpha adrenergic receptors

Question 14

what does Gas do in cAMP mediated signaling

Answer 14

stimulates adenylate cyclase in response to a variety of hormones or neurotransmitters

Question 14

what does adenylate cyclase do in cAMP mediated signaling

Answer 14

catalyzes conversion of ATP to cAMP

Question 15

what does Gai do in cAMP mediated signaling

Answer 15

inhibits adenylate cyclase from G proteins that are coupled to other GPCRs with different ligands

Question 16

what is cAMP

Answer 16

second messenger with numerous signaling roles

Question 17

what does cAMP do

Answer 17

activated PKA protein kinase A

Question 18

how is cAMP broken down to 5’AMP

Answer 18

phosphodiesterase

Question 19

PKA has what 2 subunits

Answer 19

2 regulatory and 2 catalytic

Question 20

what happens when cAMP bind to the R subunits

Answer 20

C subunits dissociate from the R subunits