BGM1004/L04 Bacterial Genetics III

Question 1

What is a genome?

Answer 1

Total of all genetic information in an individual organism

Question 2

What is genomics?

Answer 2

Acquisition, storage, retrieval and analysis of DNA sequence data

Question 3

Who created Next Generation Sequencing?

Answer 3

Fred Sanger

Question 4

How many bases can be generated in a single experiment using NGS?

Answer 4

1 million (30Gb)

Question 5

Give 3 properties of E. coli as a model organism.

Answer 5

Grows rapidly
Simple nutritional requirements
Well-established genetics

Question 6

How large is the E. coli K-12 genome?

Answer 6

4.6Mbp

Question 7

How long does it take for E. coli to complete binary fission?

Answer 7

1 hour
40 mins for C period
20 mins for D period

Question 8

How are genes with related function generally disributed in E. coli?

Answer 8

Clustered together on same chromosome

Question 9

How are chromosomes structured in Strepomyces coelicolor? (3)

Answer 9

Linear chromosomes
Core region contains main genes
Arms contain more variable regions

Question 10

What is an annotation file?

Answer 10

Data about a given organism
-1500 pages long

Question 11

What is required to convert an annotation file into a more understandable format?

Answer 11

Visualisation program

Question 12

What program can be used to compare genomes?

Answer 12

Artemis

Question 13

What are related genes described as?

Answer 13

Regions of synteny