1.5 Oxidation of pyruvate to Acetyl-CoA

Question 1

Draw the reaction of pyruvate → Acetyl-CoA

Answer 1

Question 2

Give the co-factors necessary for Pyruvate → AcCoA

Answer 2

1. HS-CoA
2. FAD
3. NAD⁺
4. Lipoic acid
5. TPP (Thiamine pyrophosphate) (Vit B₁)

Question 3

Function of TPP as a cofactor

Answer 3

Removes CO₂

Question 4

Function of NAD⁺ & FAD as cofactors

Answer 4

Oxidation

Question 5

Function of lipoic acid as a cofactor

Answer 5

HSCoA → AcCoA

Question 6

Localisation of pyruvate → Acetyl-CoA

Answer 6

Pyruvate moves into the mitochondrion

Question 7

Importance of Pyruvate → AcCoA

Answer 7

• Preparatory step of the citrate cycle
• Used in FA synthesis

Question 8

Glycerol phosphate shuttle - Function

Answer 8

• NADH + H⁺ cannot be transported from the cytoplasm → mitochondrion
• Shuttle process solves this
• Worth 2 ATP in Glycolysis

Question 9

Draw the glycerol-3-phosphate shuttle

Answer 9