GGT

Question 1

Gamma-glutamyltransferase (gamma-GT/GGT), also called

Answer 1

gamma-glutamyltranspeptidase

Question 2

gamma-glutamyltranspeptidase, is an enzyme present in

Answer 2

Liver and bile duct

Question 3

measurement of gamma-GT is widely used to rule out a

Answer 3

Hepatic or biliary origin

Question 4

valuable tool for the differential diagnosis in liver diseases.

Answer 4

Together with other enzymes such as alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and cholinesterase gamma-GT

Question 5

Method GGT

Answer 5

Kinetic photometric test according to Szasz/Persijn

Question 6

GGT PRINCIPLE

Answer 6

Gamma-GT catalyzes the transfer of glutamic acid to acceptors like glycylglycine in this case.
This process releases 5-amino-2-nitrobenzoate which can be measured at 405 nm. The increase in absorbance at this wavelength is directly related to the activity of gamma-GT.

Question 7

GGT REACTION

Answer 7

L-Gamma-glutamyl-3-carboxy-4-nitranilide + Glycylglycine
< Gamma-GT >
Gamma-glutamyl-glycylglycine + 5-Amino-2-nitrobenzoate

Question 8

R1

Answer 8

TRIS ph 8.28 (135 mmol/L)
Glycylglycine 135 mmol/L

Question 9

R2

Answer 9

L-Gamma-glutamyl-3- carboxy-4-nitroanilide ph 6.00 (22 mmol/L)

Question 10

Warnings and Precautions

Answer 10

1. The reagents contain sodium azide (0.95 g/L) as preservative. Do not swallow! Avoid contact with skin and mucous membranes.
2. In very rare cases, samples of patients with gammopathy might give falsified results [8].
3. Please refer to the safety data sheets and take the necessary precautions for the use of laboratory reagents. For diagnostic purposes, the results should always be assessed with the patient’s medical history, clinical examinations and other findings.
4. For professional use only!

Question 11

Wavelength

Answer 11

405 nm

Question 12

Optical path

Answer 12

1cm

Question 13

Temperature

Answer 13

37 degrees

Question 14

Measurement

Answer 14

Against reagent blank

Question 15

Subtrate start

Answer 15

Szasz. IFCC
1421. 1606
1158. 1309

Question 16

Reference Range
According to Szasz

Answer 16

Women < 32 (0.53 ukat/L)
Menz. <49U/L (0.82 ukat/L)

Question 17

Reference Range
According to IFCC

Answer 17

Female <38 U/L (0.63 ukat/L)
Male <55 U/L (0.92 ukat/L)