vector control Flashcards
what are mosquitos attracted to
-co2: Gr dependant olfactory neuron and orco too
-human odorants using the orco dependant olfactory neurons
-heat
Gr1-3 are blocked by what
deet
changing environment; temp and ph in host blood and vector
-host blood: temp up and ph down
-vector; temp down and ph up
where is cruzi yeeted out of fly
poop
traditional approaches to vector control
-spray insecticide on walla 6 months
-bed net
-bednets on wall: 1y
-insecticide house paint approx 2 yrs
true or false: bed nets are a slay
true
new approaches to vector control; modification of insect vectores
-infect vectors with wolbachia bacteria
-genetic modifications of vectors using crispr
what does wolbachia do
-bacteria that infects insects, passed onto off springs
-infected insects are poor vectors for pathogen transmission
-insects have a shorted lufe span
-transmitted through eggs of infected females
true or false: infected wolbachia males ensure non infected females have no kids due to cytoplasmic incompability
true
cytoplasmic incompability is caused by what
-wolbachia proteins: cifa and cifb
-cifb from sperm is toxic to embryo
-cifa from eggs binds and impairs cifb
-infected femaled will have the cifa so they can make infected kids that will be resistant to pathogens
cifb inhibits what
chromosome segregation during anaphase in the first mitotic division
true or false: homology directed repair causes no errors whereas non-homologous end joining causes errors
true
what od gene drive
chain reaction to insert crispr genes into the chromosome
what do you need in gene drive reaction
-plasmid
-ha1 and 2 that flank:
-cas 9 and the grna
-can add gene of choice too
gene drive uses which type of repair
homology directed repair
