Lecture 26 Microbial Taxonomy Flashcards

1
Q

Cells, organelles (like mitochondria and chloroplasts), and all major metabolic pathways evolved from ____

A

early prokaryotic cells

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2
Q

Common system of classification that has been recently debunked by phylogenetic data.

A

the Five Kingdom System

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3
Q

Common system of classification based on phylogenetic data

A

the Three Domain System

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4
Q

What are the three domains of life?

A

Bacteria, Archaea, and Eukarya

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5
Q

Endosymbiotic theory

A

the theory that cell organelles such as the chloroplast and mitochondrion arrised from the engulfing of prokaryotic bacteria

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6
Q

Information that supports the endosymbiotic theory

A

mitochondria have their own DNA, ribosomes, fission method, and have two membranes

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7
Q

Three domains: cell wall

A

archaea have no peptidoglycan, bacteria have peptidoglycan, eukarya have carbohydrates

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8
Q

Three domains: membrane lipids

A

archaea have branched carbon chains, bacteria have straight carbon chains, eukarya have straight carbon chains

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9
Q

Three domains: first amino acid in protein sysnthesis

A

archaea use methionine, bacteria use formylmethionine, eukarya use methionine

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10
Q

Taxonomy

A

the science of identifying, naming, and organizing living organisms into systems of classification

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11
Q

Why is taxonomy important?

A

provides a reference for identifying organisms, provides universal names for organisms, organizes organisms into categories

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12
Q

Phenotype

A

analysis of physical attributes

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13
Q

Examples of phenotype analysis

A

morphology, gram reaction, biochemical, nutritional, physiological, pH, antibiotic sensitibity

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14
Q

Genotypic

A

analysis of the genome

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15
Q

Examples of genotypic analysis

A

GC content, plasmid analysis, DNA fingerprinting, DNA hybridization, gene sequence analysis (whole & 16rRNA analysis, conserved genes analysis, multigene analysis)

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16
Q

Phylogeny

A

the study of the evolutionary history of organisms

17
Q

What is 16s rRNA?

A

a short strand of RNA commonly used for genotypic analysis

18
Q

Why is 16s rRNA so commonly used?

A

present in all cells and always has the same function (lines up mRNA), has enough sequence information, contains variable and constant regions, no horizontal transfer

19
Q

What do variable regions tell us?

A

closely-related species

20
Q

What do constant regions tell us?

A

distant relationships (same genus)

21
Q

Horizontal transfer

A

the movement of genes between different genomes

22
Q

What is the RDP?

A

the Ribosomal database project- a database with about 3 million 16s rRNA sequences available

23
Q

How many species on the RDP are well-characterized?

A

7,000

24
Q

How many species on the RDP are partially identified?

A

10,000

25
Q

What is the estimate for the total microbial species range?

A

10^7-10^9 (HUGE RANGE)

26
Q

How many of all microbial species can we cultivate?

A

<= 0.1%

27
Q

How many cells are in 1 mL of surface water?

A

10^5 cells/mL

28
Q

How many cells are in 1 g of surface soil?

A

10^8 cells/g