19

Question 1

themes of microbes

Answer 1

ubiquity, abundance metabolic power, frequently in communities

Question 2

syntrophy

Answer 2

microbes team up

Question 3

is there is a theromdynamically feasible reaction, microbes will

Answer 3

exploit it

Question 3

microbes impact

Answer 3

carbon cycle, nitrogen cycle, sulfur cube, phosphorus cycle, gases

Question 4

enrichment culture is used to

Answer 4

isolate and grow rare organisms with specific characteristics from mixed populations

Question 5

interdependent nutritional consortia

Answer 5

bacteria NEED each other to grow

Question 5

why is it difficult to grow certain bacteria in lab

Answer 5

difficult to replicate optimal growth conditions, unknown growth conditions

Question 6

why cultivate microbes

Answer 6

study; genome, physiology, metabolism

Question 7

microbe domestication

Answer 7

microbe does not exhibit traits found in nature

Question 8

Amplicon-based community fingerprinting example

Answer 8

amplify 16s ribosomal RNA to determine abundance of stuff in a sample

Question 9

cultivation independant methods

Answer 9

FISH, amplicon based community fingerprinting, antibodies, single cell genomics, metagenomics

Question 10

RNA tells

Answer 10

OTU (operational taxonomic units), abundance, phylotypes

Question 11

phylotypes

Answer 11

microbes Identified by sequence similarity

Question 12

genome analysis steps

Answer 12

shearing, sequencing, assembly, annotation

Question 13

proteome analysis steps

Answer 13

gel based separation, isolated tryptic digest, peptide sequencing/ mass fingerprinting

Question 14

Metabolome analysis

Answer 14

derivatization, GC-MS, identification analysis, total metabolites

Question 15

trancriptome

Answer 15

total rna, reverse that biddy