1.7 Basic Separation Techniques Flashcards
(26 cards)
A process whereby centrifugal force is used to separate solid matter from a liquid suspension
Centrifugation
Supernatant - _______
Sediment- ________
Liquid
solid
Parts of centrifugation includes:
- Head rotor
- Carriers
- Shields
- Motor
- control panel
- Breaking device
- Cover
Which of the following does not belong?
None
Rule of thumb in Centrifuge: Placing the tube by exact positioning or by the exact opposite
t or F
T
Purpose of the in Centrifue machine is for ________ Verification
a. Rotation
b. Speed
c. Specimen
b
How to verify Speed in the centrifuge?
a. tachometer
b. Strobe light
c. All of the above
d. None of the above
c
Force acting in the sample
a. Relative Centrifugal force
b. Revolution per minute
a
Speed of the centrifuge
a. Relative Centrifugal force
b. Revolution per minute
b
RCF Constant value?
)1.12 x 10 ^-5) r x rpm^2
Centrifugal force increases the with increasing:
a. Speed
b. length of the radial arm
Which of this does not belong?
None
Rotor and motor in Centrifuge is influence with RCF with Fictional force and Buoyant force
T or F
T
How to obtain better separation?
a. Increase the sepeed
b Increae the time
c None
c
w.o gel separator: how many mins for 1000g when it comes to centrifuge?
10-15 mins
w/ gel separator: how many mins for 1000g - 1300g when it comes to centrifuge?
10-15 mins
To protect biological specimens from famage caused by heating when centrigufed
a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge
a
Use for terminally labile analytes
a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge
a
Fixed at a angle
a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge
b
About to 7000rpm
a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge
b
Also known as Swining-bucket rotos have holders that swing outward in response to centrifugal force
a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge
c`
Not capable of higher speed (3000rpm, 1700g)
a. Refrigerated
b. Fixed Angle
c. Horizontal centrifuge
c
Which of the following is not a characteristic of horizontal Centrifuge
a. Specimen cups are in vertical position when at rest
b. The cups move to a horizontal position during centrifugation
c. Capable of higher speed
d. As the sample is centrifuged, the particles being sedimented travel down though the liquid to the bottom of the tube
c
It uses a very high torque and low inertia motor to spread monolayers of cells rapidly across a special slide for critical morphologic studies
Cytocentrifuge
Cytocentrifuge Advantage:
Small amount of sample is used producing e____ distributed cells
Evenly
Which centrifuge is used when rapid centrifugation of solution is needed?
a. Horizontal-head
b. Fixed angle
c. Cytocentrifuge
d. None of the above
b
