1S [LAB] Specimen Collection, PBS Preparation, WBC Differential Count Flashcards
(57 cards)
Artifactual morphologic changes in neutrophils begin to occur within ___ minutes of blood collection
30 mins
When EDTA-anticoagulated blood specimens are kept at room temperature, blood films should be made within ___ hours of collection to minimize storage artifacts
4 hrs
An in vitro phenomenon where platelets surround or adhere to neutrophils, which potentially causes pseudo-thrombocytopenia when counting is done by automated methods
Platelet satellitosis
It is the most convenient and most common method used for making peripheral blood films
Manual wedge technique
In manual wedge technique of PBS preparation, the pusher slide is held securely in the dominant hand at about a ___degree angle
30- to 45-degree angle
Wright-Giemsa stain is considered ___ because it contains both eosin and methylene blue
Polychrome stain
___ in the stain fixes the cells to the slide.
Methanol
The buffers added to the stain to maintain the specific pH
0.05 M sodium phosphate (pH 6.4)
Aged distilled water (pH 6.4-6.8)
Macroscopically, a well-stained blood film should be ___ in color
pink to purple
When the correct area has been selected, use of a back-and-forth serpentine, or “___,” track pattern is preferred to minimize distribution errors
battlement
Method of peripheral blood smear that is the simplest and most common
Two-slide or wedge method
In a proper peripheral blood smear, the film must cover ___ the length of the slide
2/3 to 3/4
Shape of a proper film in a peripheral blood smear
Finger shaped
This will accentuate poor leukocyte distribution by pushing larger cells to the very end and sides of the film
Moving the pusher slide forward too slowly
T/F: The slide may be labeled with a lead pencil on the frosted end or directly on the thinner end of the blood film
False (label should be at the thicker end of the blood film)
The thickness of the film can be adjusted by changing the ___ of the spreader slide or the ___ of spreading, or by using a ___ drop of blood
angle
speed
smaller or larger
An older technique of peripheral blood smear preparation that is now used only rarely for PBS but is sometimes still used for making bone marrow aspirate smears
Two-cover slip or Ehrlich’s two-coverglass method
Centrifuges that may be used to produce blood film that has uniform distribution of cells
Spinners
The ___ produces a uniform blood film, in which all cells are separated and randomly distributed
Spinner slide
Smears prepared using this method have white blood cells that can be easily identified at any spot in the film
Spinner method
An automated slide making and staining system
Sysmex SP-1000i
In Sysmex SP-1000i, the film is produced for every ___ seconds
30s
Prepares slide automatically from a single 90ul sample aspiration and up to 12 slides from a single sample presentation
DxH Slidemaker Stainer II Cellular Analysis System
The anticoagulant of choice for hematology cell counts and cell morphology
EDTA
