2S [LAB]: HCT

Question 1

Hematocrit is also known as ___

Answer 1

Packed cell volume (PCV) or Erythrocyte volume fraction (EVF)

Question 2

Hematocrit method commonly used in the laboratory

Answer 2

Adam’s microhematocrit method

Question 3

Coagulant of choice in HCT determination

Answer 3

Heparin/ EDTA

Question 4

Color of the ring of capillary tube that is heparinized

Answer 4

Red

Question 5

Color of the ring of capillary tube that is non-heparinized

Answer 5

Blue

Question 6

How much of the capillary tube should be filled in HCT determination?

Answer 6

2/3 or 3/4

Question 7

Which end of the capillary tube is sealed using sealing clay?

Answer 7

Colored ring eng

Question 8

The sealing clay should be at least how many mm thick?

Answer 8

4 mm

Question 9

Centrifuge conditions used in HCT determination

Answer 9

10 000 g - 15 000 g for 4-5 mins

Question 10

In HCT determination, the duplicate should agree within how many percent?

Answer 10

1%

Question 11

Uses precalibrated capillary tubes and has built-in hematocrit scales, which eliminates the need for separate reading devices

Answer 11

READACRIT centrifuge

Question 12

Reference range of HCT in males

Answer 12

40-54%

Question 13

Reference range of HCT in females

Answer 13

35-49%

Question 14

Identify the effect of the following on HCT reading:

Improper sealing of the capillary tube

Answer 14

Decreased

Question 15

Identify the effect of the following on HCT reading:

Short draw

Answer 15

Decreased

Question 16

Identify the effect of the following on HCT reading:

Specimen was not mixed properly

Answer 16

Decreased or increased

Question 17

Identify the effect of the following on HCT reading:

Insufficient centrifugation

Answer 17

Increased

Question 18

Identify the effect of the following on HCT reading:

Delay in reading results

Answer 18

Increased

Question 19

Identify the effect of the following on HCT reading:

Buffy coat of the specimen was included in the reading

Answer 19

Increased

Question 20

Identify the effect of the following on HCT reading:

Microhematocrit reader is not used properly

Answer 20

Increased or decreased

Question 21

Identify the effect of the following on HCT reading:

Trapped plasma

Answer 21

Increased by 1-3%

Question 22

In automation, how is HCT computed?

Answer 22

RBC x MCV

Question 23

Amount of plasma that still remains in the RBC portion after the microhematocrit has been spun

Answer 23

Trapped plasma

Question 24

Identify the effect of the following on HCT reading:

Blood loss

Answer 24

Temporarily low

Question 25

Identify the effect of the following on HCT reading: Fluid loss associated with dehydration

Answer 25

False increase

Question 26

Identify the effect of the following on HCT reading: Improper flushing of an IV catheter

Answer 26

Decreased

Question 27

Which calibration of Wintrobe tube is used for HCT determination?

Answer 27

10-0

Question 28

Centrifuge conditions in HCT determination using Wintrobe tube

Answer 28

3000 rpm for 30 mins

Question 29

Anticoagulant used in Haden's modification

Answer 29

1.1% sodum oxalate

Question 30

Centrifuge conditions in HCT determination using Haden's modification

Answer 30

3000 rpm for 20 mins

Question 31

When using the Haden's modification and the blood is at least 5mL, the shortcut factor is ___

Answer 31

20

Question 32

An HCT determination method that uses a tube with bulb and calibration of 1-10cm

Answer 32

Van Allen method

Question 33

Anticoagulant used in Van Allen method of HCT determination

Answer 33

1.6% sodium oxalate

Question 34

Centrifuge conditions in HCT determination using Van Allen method

Answer 34

2500 rpm for 15-30 mins

Question 35

Anticoagulant used in Sanford-Magath method of HCT determination

Answer 35

1.3% sodium oxalate

Question 36

In HCT determination, this method uses a tube that is about 5 inches long and a funnel-like mouth

Answer 36

Sanford-Magath

Question 37

Anticoagulant of choice used in Bray's method of HCT determination

Answer 37

Heparin

Question 38

An HCT determination method that uses a tube similar to Wintrobe tube

Answer 38

Bray's method

Question 39

An HCT determination that is stand at a vertical position for 1 hour

Answer 39

Bray's method