2.2.2 Biochemical test (Gram positive, Catalase Negative)

Question 1

A test that is use to differentiate Staph and micrococcus but can also used to differentiate S. pyogenes

Answer 1

Bacitracin

Question 2

In bacitracin, who is susceptible? (Gram pos, Cat neg)

Answer 2

S. pyogenes

Question 3

In bacitracin, who is Resistant? (Gram pos, Cat neg)

Answer 3

Other strept. except pyogenes

Question 4

Positive indicator for Bacitracin?

Answer 4

10mm zone of inhibition

Question 5

A test that is use to differentiate Beta hemolytic streptococcal

Answer 5

Sulfamethoxazole-Trimethoprim (SXT)

Question 6

What is the Susceptible in SXT?

Answer 6

Non “beta” hemolytic Streptococcaceae

Question 7

What is the Resistant for SXT?

Answer 7

S. pyogenes
S. agalactiae
S. dysagalactiae subsp. equisimilis

Question 8

How many grams for SXT?

Answer 8

1.25ug

Question 9

Test for the ability of the organism to hydrolyze the substrate L-pyrrolidonyl-β-napththylamide (PYR).

Answer 9

Pyrrolidonyl aminopeptidase / Pyrrolidonyl-beta-naphthylamide

Question 10

What bacteria is positive in PYR?

Answer 10

Group A
Group D Enterococcus

Question 11

What bacteria is Negative in PYR?

Answer 11

The rest
Group D non enterococcus

Question 12

Positive indicator for PYR?

Answer 12

5 mins Bright red (pink to cherry red)

Question 13

A test used for the presumptive identification of group B streptococci (S.
agalactiae), Clostridium, Listeria

Answer 13

CAMP (Christie-Atkins-Munch-Peterson)

Question 14

Test for synergistic hemolysis between group B Streptococcus and βhemolytic Staph. aureus

Answer 14

CAMP (Christie-Atkins-Munch-Peterson)

Question 15

In CAMP, what bacteria is swabbed vertically?

Answer 15

Staphylococcus aureus

Question 16

In CAMP, what bacteria is swabbed horizontally?

Answer 16

Group B strept
Listeria monocytogenes

Question 17

What is the positive indicator for CAMP?

Answer 17

Arrowhead

Question 18

In CAMP, what is being detected in Group B Strept?

Answer 18

CAMP factor

Question 19

In CAMP, if Clostridium is being identified, what bacteria is vertical?

Answer 19

S. agalactiae

Question 20

What is the positive indicator for Clostridium in CAMP?

Answer 20

Reversed arrowhead / Bowtie

Question 21

What is the positive indicator for L. monocytogenes in CAMP?

Answer 21

Black arrowhead

Question 22

It is test for hippuricase enzyme of Group B Streptococci

Answer 22

Hippurate hydrolysis

Question 23

IT hydrolyzes sodium hippurate to sodium citrate and Ninhydrin

Answer 23

Hippuricase enzyme

Question 24

What are the reagents of Hippurate hydrolysis?

Answer 24

Sodium hippurate and ninhydrine

Question 25

What is the positive indicator for Hippurate hydrolysis

Answer 25

Deep blue color

Question 26

What microorganisms is positive in hippurate hydrolysis?

Answer 26

S. agalactiae

Question 27

Detects for the ability of the bacteria to hydrolyze Leucine-β-naphthylamide

Answer 27

LAP (Leucine aminopeptidase)

Question 28

What is the reagent for LAP?

Answer 28

Cinnamaldehyde

Question 29

What organisms is positive in LAP test?

Answer 29

All viridans (S. pneumoniae and S. pyogenes, Enterococcus, Pediococcus) - ppt

Question 30

What is the positive indicator for LAP?

Answer 30

Red color in 1 min

Question 31

Detects the action of β-D-glucuronidase

Answer 31

B-D-aminopeptidase

Question 32

where can β-D-glucuronidase be found in what bacteria?

Answer 32

Large colony forming Beta hemolytic group C and G

Question 33

What is the positive for B-D-aminopeptidase?

Answer 33

G, C group

Question 34

What is the Negative for B-D-aminopeptidase?

Answer 34

Group F (Strep. anginosus)

Question 35

Detects for the ability of the bacteria to grow in the presence of 40% bile and hydrolyze esculin

Answer 35

Bile Esculin (Bile esculin hydrolysis)

Question 36

What are the reagents for bile esculin?

Answer 36

40% bile Ferric NH4 (ammonium)

Question 37

What is the positive indicator for Bile esculin?

Answer 37

Black

Question 38

What is positive for Bile esculin?

Answer 38

ALL Group D

Question 39

Test the ability of the organism to grow in 6.5% NaCl

Answer 39

Salt tolerance test

Question 40

Differentiates Group D strep

Answer 40

Salt tolerance test

Question 41

What is the positive indicator for Salt tolerance test?

Answer 41

Turbidity and color change (After 18-24 hours)

Question 42

What is positive in Salt tolerance test?

Answer 42

Group D enterococcus

Question 43

What is Negative in Salt tolerance test?

Answer 43

Group D non-enterococcus

Question 44

Known as Taxo P (Ethylhydrocupreine hydrochloride) that differentiates S. pneumoniae from alpha hemolytic

Answer 44

Optochin Susceptibility

Question 45

how many grams of Ethylhydrocupreine hydrochloride in optochin susceptibility test?

Answer 45

5ug

Question 46

What is the Susceptible indicator for optochin susceptibility?

Answer 46

14 or 16 mm zone of inhibition

Question 47

What is the Resistant indicator for optochin susceptibility?

Answer 47

<14 or 16 mm zone of inhibition

Question 48

What is organisms is susceptible in Optochin sus?

Answer 48

S. pneumoniae

Question 49

Confirmation test in determining the alpha hemolytic Differentiates S. pneumoniae and viridans

Answer 49

Bile solubility test

Question 50

2 methods of bile solubility test

Answer 50

BAP Broth

Question 51

What reagent is used in bile solubility?

Answer 51

Sodium deoxycholate

Question 52

How man Percent in BAP for sodium deoxycholate?

Answer 52

10%

Question 53

How man Percent in Broth for sodium deoxycholate?

Answer 53

2%

Question 54

What is the positive indicator for BAP in Bile solubility?

Answer 54

Clear and lyse colony

Question 55

What is the negative indicator for BAP in Bile solubility?

Answer 55

Intact colony

Question 56

What is the positive indicator for broth in Bile solubility?

Answer 56

Clear

Question 57

What is the Negative indicator for broth in Bile solubility?

Answer 57

turbid

Question 58

What is soluble in Bile solubility?

Answer 58

S. pneumoniae

Question 59

What is insoluble in Bile solubility?

Answer 59

Viridans

Question 60

What are the Test for Group A?

Answer 60

Bacitracin PYR

Question 61

What are the Test for Group B?

Answer 61

CAMP Hippurate hydrolysis

Question 62

What are the Test for Viridans and S. pneumoniae?

Answer 62

Optochin susceptibility Bile solubility

Question 63

What are the Test for Group D?

Answer 63

PYR Bile esculin Salt tolerance (6.5%)

Question 64

What are the Test for Streptococcal like organisms?

Answer 64

LAP