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Bacte Lec (Overview) [Prelims 1] > 3.1.5 Lab Diagnosis > Flashcards

3.1.5 Lab Diagnosis Flashcards

(71 cards)

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1
Q

What are the specimen use for N. gonorrhoeae?

A

Genitalia: Urethra, cervix

Rectum and pharynx

Eye discharge

Blood/Joint fluids

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2
Q

How many cm is used to insert in the rectum?

A

4-5 cm

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3
Q

How many cm is used to insert in the Urethra?

A

2 cm while slowly rotating

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4
Q

Why you shouldn’t use cotton with fatty acid?

A

Inhibit growth of N. gonorrhoeae

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5
Q

What are the 4 swabs?

A

Dacron swab
Polyurethane swab
Rayon swab
Flocked nylon swab

(DaPoRaF)

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6
Q

What is the best swab use that does not contain fatty acid?

A

Dacron swab
Rayon swab

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7
Q

What specimen is used for Ophthalmia neonatorum

A

Eye discharge

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8
Q

Where is blood/join fluid is used?

A

Disseminated gonococcal infection

Gonococcal arthritis

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9
Q

What is the ideal specimen for N. meningitidis?

A

Cerebrospinal fluid

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10
Q

How to improve detection of CSF?

A

Increase concentration

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11
Q

What is the amount of required CSF?

A

1 mL at 1000xg for 10 mins in Centrifuge

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12
Q

What are the other specimen use for n. meningitidis?

A

Nasopharyngeal swabs and aspirates

Blood

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13
Q

What are the specimen use for M. catarrhalis?

A

Middle ear effusion
Nasopharynx
Sinus aspirates
Sputum
Bronchial aspirates

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14
Q

Cotton swabs contains a reagent that inhibits the growth of N. gonorrhoeae

A

Calcium alginate

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15
Q

What are the transport media use for Neisseria gonorrhoeae?

A

Transgrow
JEMBEC
Bio-bag
Gono-Pak
Amies medium

(BAG JT)

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16
Q

When using Amies medium within how many hours should be plated?

A

6 hours

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17
Q

What is the selective media for Neisseria sp.?

A

Chocolate agar plate

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18
Q

In direct microscopic examination, what is usually the specimen?

A

Urogenital/Eye discharge

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19
Q

What is the shaped of N. gonorrhoeae and meningitidis

A

kidney/coffee bean shape

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20
Q

The most commonly seen N. gonorrhoeae is called?

A

Intracellular gram negative diplococci

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21
Q

N. gonorrhoeae is can be seen in both intracellular and extracellular

A

TRUE

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22
Q

Moraxella catarrhalis can only be seen Intracellularly?

A

TRUE

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23
Q

What is the test for M. catarrhalis?

A

Penicillin disk test

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24
Q

REVIEW

A

If you see a gram-negative rod, it might be Kingella spp. If it’s an
elongated gram-negative rod, it is Acinetobacter spp.

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25
What is the culture media of choice for Neisseria?
Chocolate Agar plate
26
What is the composition for thayer Martin agar?
VCN (Vancomycin, Colistin, Nystatin)
27
What is the function of Vancomycin?
Inhibit gram positive
28
What is the function of Colistin?
Inhibit gram negative
29
What is the function of Nystatin?
Inhibit yeast cell
30
What is the composition for Modified Thayer Martin agar?
- VCN - Trimethoprim lactate
31
What is the function of Trimethoprim lactate?
Inhibit swarming Proteus
32
What is the composition for Martin Lewis agar?
VCT (Vancomycin, Colistin, Trimethoprim lactate) - Anisomycin
33
What is the function of Anisomycin?
Inhibits yeast cell
34
What is the composition for New York City agar?
- VCT - Amphotericin B
35
What is the function of Amphotericin B?
Inhibits yeast
36
What is the composition for GC agar?
- VCT - Amphotericin B - Lincomycin
37
What is the function of Lincomycin?
Inhibits Gram Positive
38
What is the most commonly used culture media for Neisseria sp?
Thayer Martin
39
What is the Temp in the incubation for Neisseria and how long it will be?
35C in 72 hrs 3-5% CO2
40
Where is the Neisseria media being place before incubation?
Candle jar
41
What type of candle jar should be used?
White unscented
42
What is the characteristics of N. gonorrhoeae in Agar plate?
Small tan Translucent Raised
43
If we see in the gram-stain more than 5 WBCs and/or pus cells BUT without the presence of any bacteria AT ALL, What is this hidden bacteria?
Chlamydia trachomatis Europlasma eurolyticum
44
What is the characteristics of N. meningitidis in Agar plate?
Small tan mucoid convex
45
N. meningitidis can grow on both BAP and CAP?
TRUE
46
What is the characteristics of M. catarrhalis in Agar plate?
Smooth opaque gray to white
47
What are the Biochemical test for Neisseria?
Oxidase test Superoxal test Carbohydrate utilization Dnase Butyrate Esterase test
48
Determines presence of the cytochrome oxidase system that oxidizes cytochrome with molecular oxygen
Oxidase test
49
Where is oxidase test usually test on?
Filter paper Directly on plate
50
What is the main reagent of oxidase test?
Tetramethyl-p-phenylene-diamine-dihydrochloride
51
What is the positive indicator for Oxidase test?
Purple / Deep purple
52
What is the test that uses 30% hydrogen peroxide?
Superoxol catalase test
53
What is the positive indicator for Superoxol catalase test?
Bubbles
54
What only bacteria is positive on 30% hydrogen peroxide?
N. gonorrhoeae
55
All Neisseria is positive on 3% hydrogen peroxide except?
elongata bacilliformis
56
What is the media for Carbohydrat Utilization test?
Cystine trypticase agar
57
Cystine trypticase agar contains 1% of what carbs?
Glucose Maltose Lactose Fructose Sucrose
58
Cystine trypticase agar contains carbs that are in different tubes?
TRUE
59
What is the pH indicator for Cysteine trypticase agar?
Phenol red
60
What is the positive indicator for Cysteine trypticase agar?
yellow
61
How cysteine trypticase agar turns carbs into yellow?
In anaerobic environment, it will change to acid
62
All Neisseria are GLUCOSE fermenter except?
flavescens M. catarrhalis
63
N. flavescens and M. catarrhalis are?
Asaccharolytic - does not breakdown sugar in anaerobic environment
64
What bacteria is only positive in GLUCOSE?
N. gonorrhoeae
65
What bacteria is only positive in - Glucose - Maltose
N. meningitidis
66
What bacteria is only positive in - Glucose - Maltose - LACTOSE
N. lactamica
67
What bacteria is only positive in - Glucose - Maltose - Sucrose - Fructose
N. sicca
68
What bacteria is positive for Dnase test?
M. catarrhalis
69
What test that adds colony then substrate that is similar to oxidase test?
Butyrate Disk / Tributyrin hydrolysis
70
What is the positive indicator for Butyrate disk?
Blue
71
What bacteria is positive to Butyrate test?
M. catarrhalis

Bacte Lec (Overview) [Prelims 1] (34 decks)

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