Flashcards in 3 Antibody Diversity and B Cell Development Deck (47):
What is a B cells purpose?
Where are B cells made?
What is the terminal B cell differential cell that makes antibodies?
What is the long lived antigen specific cell ready to become an antibody producing cell?
Memory B Cell
What process in somatic recombination does not rely on antigen?
Somatic Recombination: Heavy and Light Chain Rearrangement
How is diversity in the B cell repertoire created?
Different gene splicing
Imprecise protein joining
Different protein pairing
What are light chain genes?
What chromosome is Kappa light chain gene found on?
What chromosome is Lambda light chain gene found on?
What regions do each Kappa and Lambda gene have?
V, J, Constant regions
What regions do heavy chain genes have?
V,D,J, C regions
How many heavy chain Constant gene alleles are there?
9, one for each Ig type
What brings the V,D, and J segments together?
What brings the C region onto the VDJ or VJ segments?
What do leader sequences do?
Direct Ig to:
How many alleles of each chain sub type are expressed?
Only 1, or the cell dies
What parts of B cell diversity creation are antigen dependent?
Where does Somatic Hypermutation occur?
What does Somatic Hypermutation do?
Creates Affinity Maturation
What B cell undergoes Somatic Hypermutation and Class Switching?
Activated B cell
What are CDRs?
Complementary determining regions (hypervariable adaptive regions of Somatic Hypermutation Igs)
What B cell function relies on Helper T cells to occur?
What gene segment changes in class switching?
* Vh exon associates with Ch genes to change the type of Constant region that will get expressed. This changes the type of Ig that is made.)
What Ig is made in every cell initially?
What Class Switch cannot occur?
IgM to IgD
What regions occur between V and C regions to allow class switching?
What enzyme allows class swithcing inside the DNA of B cells?
What cells do B cells depend on to live and grow?
Where do stromal cells live?
What is the overview of B cell development?
1. Pro B cell
2. Pre B cell
3. Immature B cell
4. Mature B cell
What is indicative of early Pro B cells?
DJ gene rearrangement
What is indicative of late Pro B cells?
V-DJ gene rearrangement
What is indicative of early Pre B cells?
m-chain expressed on surface
What is indicative of late Pre B cells?
What is indicative of Immature B cells?
Clonal deletion of:
What is indicative of Mature B cells?
Surface expression of:
What occurs if Mature B cells do not present IgM or IgD?
Apoptosis via CD8
What gene product allows double strand dna break and somatic recombination?
What enzyme allows the addition of N nucleotides at the junction of rearranged gene segments?
What is involved in surface receptor signal transduction? What disease does this lead to?
Bruton's thymidine kinase (Btk)
X linked agammaglobulinemia
What enzyme allows class switching?
What 3 things create self selection of B cells?
1. self recognition of surface molecules
2. self recognition of soluble molecules
3. inability to bind antigen
What is vital to maturation of B cells?
What do B cells present to CD4?
MHC II : Antigen complex
What signal does CD4 send back to B cells to feedback for differentiation?
What is the difference between membrane-bound Ab and secreted Ab?