3 Antibody Diversity and B Cell Development Flashcards Preview

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Flashcards in 3 Antibody Diversity and B Cell Development Deck (47):
1

What is a B cells purpose?

Make antibodies

2

Where are B cells made?

Bone Marrow

3

What is the terminal B cell differential cell that makes antibodies?

Plasma Cell

4

What is the long lived antigen specific cell ready to become an antibody producing cell?

Memory B Cell

5

What process in somatic recombination does not rely on antigen?

Somatic Recombination: Heavy and Light Chain Rearrangement

6

How is diversity in the B cell repertoire created?

Different gene splicing
Imprecise protein joining
Different protein pairing

7

What are light chain genes?

Kappa
Lambda

8

What chromosome is Kappa light chain gene found on?

Chromosome 2

9

What chromosome is Lambda light chain gene found on?

Chromosome 22

10

What regions do each Kappa and Lambda gene have?

V, J, Constant regions

11

What regions do heavy chain genes have?

V,D,J, C regions

12

How many heavy chain Constant gene alleles are there?

9, one for each Ig type

13

What brings the V,D, and J segments together?

DNA splicing

14

What brings the C region onto the VDJ or VJ segments?

RNA splicing

15

What do leader sequences do?

Direct Ig to:
Secrete
Membrane

16

How many alleles of each chain sub type are expressed?

Only 1, or the cell dies

17

What parts of B cell diversity creation are antigen dependent?

Somatic Hypermutation
Class Switching

18

Where does Somatic Hypermutation occur?

Secondary lymph

19

What does Somatic Hypermutation do?

Creates Affinity Maturation

20

What B cell undergoes Somatic Hypermutation and Class Switching?

Activated B cell

21

What are CDRs?

Complementary determining regions (hypervariable adaptive regions of Somatic Hypermutation Igs)

22

What B cell function relies on Helper T cells to occur?

Class Switching

23

What gene segment changes in class switching?

Constant (
* Vh exon associates with Ch genes to change the type of Constant region that will get expressed. This changes the type of Ig that is made.)

24

What Ig is made in every cell initially?

IgM

25

What Class Switch cannot occur?

IgM to IgD

26

What regions occur between V and C regions to allow class switching?

Switch regions

27

What enzyme allows class swithcing inside the DNA of B cells?

Switch recombinase

28

What cells do B cells depend on to live and grow?

Stromal Cells

29

Where do stromal cells live?

Bone Marrow

30

What is the overview of B cell development?

1. Pro B cell
2. Pre B cell
3. Immature B cell
4. Mature B cell

31

What is indicative of early Pro B cells?

DJ gene rearrangement

32

What is indicative of late Pro B cells?

V-DJ gene rearrangement

33

What is indicative of early Pre B cells?

m-chain expressed on surface

34

What is indicative of late Pre B cells?

V-J rearrangement

35

What is indicative of Immature B cells?

Clonal deletion of:
Anergics
Self Recognizers

36

What is indicative of Mature B cells?

Surface expression of:
IgM
IgD

37

What occurs if Mature B cells do not present IgM or IgD?

Apoptosis via CD8

38

What gene product allows double strand dna break and somatic recombination?

RAG 1
RAG 2

39

What enzyme allows the addition of N nucleotides at the junction of rearranged gene segments?

TdT

40

What is involved in surface receptor signal transduction? What disease does this lead to?

Bruton's thymidine kinase (Btk)
X linked agammaglobulinemia

41

What enzyme allows class switching?

switch recombinase

42

What 3 things create self selection of B cells?

1. self recognition of surface molecules
2. self recognition of soluble molecules
3. inability to bind antigen

43

What is vital to maturation of B cells?

Antigen!

44

What do B cells present to CD4?

MHC II : Antigen complex

45

What signal does CD4 send back to B cells to feedback for differentiation?

CD40L

46

What is the difference between membrane-bound Ab and secreted Ab?

Membrane is:
Monomeric
Transmembrane domain

47

Secretory and Membrane bound Ab pathways are allowed by:

RNA processing