Diagnostic Microbiology

Question 0

True or false. Establishing maximum inhibitory conc is part of susceptibility testing of isolate

Answer 0

False.minimum, not maximum

Question 1

Set of strains within particular species

Answer 1

Type

Question 2

Basis of classical methods of bacterial separation

Answer 2

Metabolic & Morphological characteristics

Question 3

Basis of modern taxonomic approach

Answer 3

Bacteria’s structural composition

Question 4

Comparison of species does not involve comparison of multiple strains for each species. True or false?

Answer 4

False

Question 5

Hybridization and sequencing are process of __analysis

Answer 5

Molecular

Question 6

What is DNA-DNA homology?

Answer 6

How well 2 diff DNA strands hybridize

Question 7

Purpose of DNA-DNA homology

Answer 7

To test genetic relatedness

Question 8

Sequencing of ___ : “gold standard” in bacterial taxonomy

Answer 8

16S rRNA

Question 9

16S rRNA provides complementary info for DNA-DNA homology. True or false?

Answer 9

True

Question 10

If sample has no prior culture, how can the antigen be detected?

Answer 10

Aggregation (to antibody-coated latex beads)

Question 11

Process used to amplify bacterial DNA from human fluid

Answer 11

Polymerase chain reaction

Question 12

Serologic test can be done even before infection. True or false?

Answer 12

False. Several weeks after dapat

Question 13

Sequence the five I's
A. Inspection
B. Incubation
C. Isolation
D. Identification
E. Inoculation

Answer 13

E B C A D

Question 14

True or false. No microbes require live organism as inoculation media

Answer 14

False. Some do (animal/egg)

Question 15

Identify:

• catalase positive
• Gram positive in chains
• strictly aerobic

Answer 15

Micrococcus

Question 16

All are common bet Neisseria, Moraxella and Branhamella except

a) Gram negative cocci
b) positive in both oxidase and catalase
c) facultative anaerobe

Answer 16

c) facultative anaerobe

aerobic kasi

Question 17

Identify:

• catalase negative
• anaerobic
• oxidase negative
• strictly aerobic

Answer 17

Veilonella

Question 18

All are common bet Bacillus and Clostridia except

a. sporeformer
b. gram + rod
c. aerobic

Answer 18

c. aerobic

anaerobes ang Clostridia

Question 19

All are common bet Mycobacterium and Nocardia except

a. acid-fast
b. forms spores
c. rods

Answer 19

b. forms spores

Question 20

common traits of Listeria and Lactobacillus

Answer 20

gram positive, non-sporeforming rods

Question 21

common traits of Corynebacterium and Propionibactrium

Answer 21

gram positive, pleomorphic, nonsporeforming rods

Question 22

Sequence the acid fast stain procedure

a. methylene blue
b. carbol fuschin
c. acid alcohol

Answer 22

B C A

Question 23

semi-solid media is often used for __, __

Answer 23

demonstration of motility, anaerobe isolation

Question 24

Identify mismatched pair of selective media

a. MacConkey: gram positive
b. Sabouraud: fungi
c. Lowenstein Jensen - Mycobacteria
d. MSA - Staph

Answer 24

a. MacConkey: gram positive

negative dapat

Question 25

differentiate capsule from slime layer

Answer 25

mas defined ang capsule

Question 26

True or false: capsules are not lost during in vitro culture

Answer 26

false

Question 27

Wall-less bacteria that cannot replicate but have outer membrane

Answer 27

spheroplasts

Question 28

wall-less bacteria that cannot replicate and do not have outer memebrane

Answer 28

protoplasts