Bordetella

Question 0

False about Bordetella pertussis
A. Transmission by aerosolized droplets
B.Non-invasive to cells or tissue
C. May also affect other animals

Answer 0

C

It affects humans only

Question 1

False about Bordetella
A. Strict anaerobe
B. Non motile
C. ferments glucose and lactose (no gas from both)
D. Resemble H. influenzae •

Answer 1

A

Strict aerobe

Question 2

B pertussis grows small, hemolytic colonies on __

Answer 2

Bordet Gengou medium

It does not grow on BAP and MAC

Question 3

Identify
• causes diseases in animals such as kennel cough in dogs and snuffles in rabbits
• occasionally causes respiratory disease

Answer 3

Bordetella bronchiseptica

Question 4

Which is false?
A) B parapertussis & B bronchoseptica grow on SBP/MAC
B) B parapertussis & B bronchoseptica are urease positive
C) B pertussis & B bronchoseptica are oxidase positive; B parapertussis is oxidase negative
D) All three medically impt Bordetella species are non-motile

Answer 4

D

B bronchoseptica is motile

Question 5

Preferred specimen for Bordetella diagnosis

Answer 5

saline nasal wash

Question 6

Which does not happen in direct fluorescent antibody test?
A)FA reagent can be used to examine nasopharyngeal swab specimens B)false-positive and false-negative results may occur
C)most useful in identifying B pertussis

Answer 6

C

only useful after culture on solid media

Question 7

Bordetella virulence genes, bvgA and bvgS. What does each one do?

Answer 7

• bvgS responds to environmental signals while bvgA is a transcriptional activator of the virulence genes

Question 8

What is cell chorum?

Answer 8

organisms when they multiply in the body, if they have enough number they will secrete virulence

Question 9

Enumerate Stages of whooping cough

Answer 9

Catarrhal
Paroxysmal
Convalescent

Question 10

A.Profuse and mucoid rhinorrhea (runny nose) that persists for 1 to 2 weeks.
B.Nonspecific findings such as malaise, fever, sneezing, and anorexia may also be present.
C.The disease is most communicable at this stage, since it develops the whoop.

Which is false about catarrhal stage?

Answer 10

C

Whoop @ paroxysmal

Question 11

False about paroxysmal stage
A.Appearance of a persistent cough with the characteristic “whoop” upon inhalation
B.This leads to rapid exhaustion and may be associated with vomiting, cyanosis, and convulsions
C.Runny nose also present

Answer 11

C

Sa catarrhal madalas yung runny nose.

Question 12

In whooping cough, there is T Lymphocytosis. What is T lymphocytosis?

Answer 12

T Lymphocytes promoted/activated but impaired chemotaxis or do not proliferate

Question 13

Identify the adhesin
• surface molecule
• rod-like protein that agglutinate erythrocytes.
• Mediates adhesion with an amino acid sequence (arginine, glycine, aspartic acid) present in host integrins, epithelial cells, and macrophages.

Answer 13

Filamentous hemagglutinin

Question 14

Identify the virulence factor

• Required for PERSISTENT tracheal colonization (involved in attachment)
• Component of some acellular pertussis vaccines
• Required for protective immunity to infection

Answer 14

Pili/fimbriae

Question 15

The filamentous hemagglutinin and pertussis toxin are secreted proteins and are found inside of the B. pertussis cells.
T or F?

Answer 15

F. Outside dapat

Question 16

Identify the pertussis toxin

• interferes with cellular signaling, chemotaxis, superoxide generation, and microbicidal function of immune effector cells
• calmodulin-activated with adenylate cyclase and hemolysin activity
• increase in CAMP –> imbalance of ions damages the cell

Answer 16

Adenylate cyclase toxin

Question 17

Identify the pertussis toxin

• Heat-labile secreted toxin
• Transglutaminase activity that acts on small GTPases of the Rho family
• Induces localized necrosis

Answer 17

Dermonecrotic toxin

Question 18

• Disaccharide-tetrapeptide monomeric by-product of peptidoglycan synthesis - Inhibits DNA sythesis in ciliated cells and is not regulated by bvg
• Increased IL-1 and nitric oxide production

Identify the pertussis toxin

Answer 18

Tracheal cytotoxin

Question 19

Identify

• Allows Bordetella to translocate effector proteins directly into host cells - Required for PERSISTENT tracheal colonization
• Inhibits host immune response
• Induces necrotic cell death

Answer 19

Type III secretion system

Question 20

False about lipooligosaccharide
A.Activate and induce TNF production in macrophages 
B.with repetitive O-antigen structure 
C.pyrogenic
D.mitogenic

Answer 20

B

lack of dapat

Question 21

Which is not a systemic effect of pertussis toxin?

1. T cell Lymphocytosis with ↓ mitogenicity
2. ↑ insulin secretion
3. Histamine sensitization
4. ↑ IgM production

Answer 21

4

IgE dapat

Question 22

ribosylation of G proteins inhibits _____

Answer 22

Signal transduction

Question 23

Identify
substances which prolong the exposure to the antigen; coats the antigen so it will not degrade easily, so it will persist; enhances immune response

Answer 23

Adjuvant

Question 24

A.B pertussis survives for long periods outside the human host.
B.There are no vectors.
C.Transmission is largely by the respiratory route from early cases and possibly via carriers.

Which is false?

Answer 24

A

Short period dapat

Question 25

The bacteria liberate the toxins and substances that irritate surface cells, causing _____ and ____

Answer 25

The bacteria liberate the toxins and substances that irritate surface cells, causing coughing and marked lymphocytosis.

Question 26

Obstruction of the smaller bronchioles by mucous plugs results in atelectasis and diminished oxygenation of the blood. This may lead to what clinical manifestation?

Answer 26

Convulsions in infants

Question 27

Effective antibiotic vs B pertussis

Answer 27

Erythromycin