15. Pathogenic E. coli Flashcards
How many serotypes of pathogenic E. coli?
> 700, based on cell surface antigens
3 types of diseases caused by pathogenic E. coli?
- Intestinal disease (gastroenteritis)
- UTIs
- Neonatal meningitis
5 types of pathogenic E. coli that can cause intestinal disease?
HPTIAgg
Entero..
EHEC (hermorrhagic) EPEC (pathogenic) ETEC (toxigenic) EIEC (invasive) EAggEC (aggregative)
Which type of pathogenic E.coli causes traveler’s diarrhea?
EPEC (pathogenic)
Which type of pathogenic E.coli affects people the most?
EHEC
Pathogenic E.coli transmission and duration?
Contaminated food, ppl-to-ppl
Usually 7 days
2 strategies E. coli 0157:H7 uses to create diarrhea?
- Injected effectors
2. Shiga toxin
Why would a pathogen want to cause diarrhea?
To disseminate into the environment
4 mechanisms of diarrhea generation by pathogenic E. coli?
- Breach of tight junctions
- Alteration of water channels (aquaporins)
- Alteration to serotonin functions
- Alteration of ion channels
What does pathogenic E.coli do to tight junctions that will eventually cause host diarrhea?
E.coli secretes bacterial effector proteins (EspF, EspG, Map)
E.coli secreted protein and mitochondria affected protein
How does the alteration of water channels cause diarrhea?
Only a single water molecule can pass through the channel at a time
Also uses EspF and EspG which rips water channels out
Removes AQP2 and AQP3 from the membrane
How does the alteration of serotonin functions cause diarrhea?
Works on the nervous system of the intestine
How does the alteration of ion channels cause diarrhea?
Shiga toxins attach to receptors => cause signaling cascades that alter the functions of ion channels => ions and water are released from the cell
Why would the host have trouble detecting pathogens being secreted from E. coli?
E. coli T3SS needle shields them
Pathogenic E. coli is a _____cellular pathogen
extracellular