diagnostics Flashcards

1
Q

What is the animal immunized with to create antibodies

A

innactivated pathogens

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2
Q

What are monoclonal antibodies used to detect

A

Pathogens

Antibodies

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3
Q

What are monoclonal antibodies made from

A

mouse antibody produced by a single clone of b cells

specificity for a single epitope

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4
Q

Direct immunofluorescent antibody test

A

detection o f pathogen-specific antigens in tissues or blood smears
Diagnosis of infections
Specific for pathogens

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5
Q

What is a disease that direct immunofluorescent antibody test is used for

A

FeLV

Rabies

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6
Q

Indirect immunofluorescent antibody test

A

Detection of pathogen-specific antibody
Usually serum
Patient’s IgG

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7
Q

Difference between indirect and direct immunofluorescent antibody test

A

Direct- Specific antigen

Indirect- Specific antibody

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8
Q

What is a disease indirect immunofluorescent antibody test can be used to detect

A

ehrlichia canis from ticks

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9
Q

What is ELISA

A

enzyme-linked immunosorbent assay

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10
Q

what does ELISA test for

A

test for antigens or antibodies in patient samples

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11
Q

Antigen capture assay

A

Type of ELISA

Detect pathogens

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12
Q

Antibody capture assay

A

Type of ELISA used to Detect antibodies

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13
Q

What is in antigen capture ELISA well

A

Capture Ab to Agent X
Patient serum with agent X
HRP-detection Ab for agent X
HRP substrate

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14
Q

What’s in an anitbody capture ELISA well

A

Capture Ag
Patient serum with Ab to agent X
HRP-detection Ab
HRP substrate

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15
Q

What’s one problem with antibody ELISA

A

can’t differentiate between infected and immunized

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16
Q

Polyclonal antibodies

A

Raised in an animal to detect pathogens

17
Q

Hemagglutination

A

cross linking of RBC’s

18
Q

What are 2 hemagglutination assays

A

Coombs test

Blood typing

19
Q

What does Coombs test detect

A

antibodies to RBC

20
Q

What does hemagglutination assays detect

A

RBC antigens

determine blood type

21
Q

What does hemagglutination inhibition assay measure

A

Antibodies to viruses that naturally agglutinate RBC’s

22
Q

What test do you use for canine parvovirus

A

Hemagglutination inhibition assay

23
Q

What is serum neutralization assay used for

A

detection of virus specific antibodies

24
Q

What types of viruses are serum neutralization assays used for

A

viruses that don’t agglutinate RBC

25
Q

What are antibody titer assays used for

A

estimate the amount of pathogen-specific Ab in serum

26
Q

Antibody titer

A

reciprocal of the highest serum dilution that gives a positive result in the test