Exam questions-Crawford

Question 1

What innate response provides a first line of defense against bacterial infectiion

Answer 1

activation of the alternate complement pathway by the bacterial surfaces

Question 2

What is the most important diference between the classical and alternate complement pahtways

Answer 2

the classical pathway is activated by antigen:antibody complexes

Question 3

If an animal has a C3 deficiency, what would be the most common clinical sign

Answer 3

Recurrent bacterial infections

Question 4

What is the general purpose of immunodiagnostic assays

Answer 4

Detection of an antigen or antibody in patient samples

Question 5

What test will be performed on an animal suspected of rabies

Answer 5

direct immunoflourescent antibody test for rabies virus antigens in the brain tissue

Question 6

What test do you conduct to see if antibodies are in the blood

Answer 6

indirect immunoflourescent antibody test

Question 7

What is the correct combination of reagents to use in an ELISA to detect antibodies

Answer 7

EIA antigen to coat wells, patient serum, anti-(animal) IgG antibody conjugated to HRP as the detection antibody, HRP substrate

Question 8

What is the correct combination of reagents to use in an ELISA to detect antigne

Answer 8

anti-(disease) antibody to cat wells, patient serum, anti-(disease) antibody conjugated to HRP as the detection antibody, HRP substrate

Question 9

can you test for both an antigen and an antibody in the same test

Answer 9

no, the capture antibody will bind the capture antigen

Question 10

What would you add to a serum sample in order to capture heartworm antigen, FeLV antigen, and FIV antibody

Answer 10

mouse anti-heartworm antibody conjugated to HRP, mouse anti-FeLV antibody conjugated to HRP, and FIV antigen conjugated to HRP

Question 11

What complement path is activated by binding IgG to antigens on cell surface

Answer 11

Classical pathway

Question 12

What complement pathway is directly activated by bacteria in the absence of a humoral immune response

Answer 12

alternate pathway

Question 13

What is the key enzyme formed by both the classical and alternate complement pathways that allows for the completion of the cascade

Answer 13

C3 convertase

Question 14

What is the key protein generated by this enzyme that coats bacterial surfaces and infected cell surfaces

Answer 14

C3b

Question 15

What is the most important effector functions that result from complement activation by bacterial infection

Answer 15

lysis of bacteria, phagocytosis of bacteria, inflammation

Question 16

How would you test a blood sample for the presence of antigens from an infectious agent

Answer 16

direct imunofluorescent antibody test that uses the patient’s blood smear as a substrate and FITC-labeled antibody specific for agent