9. HPV (papillomaviruses)

Question 1

Genome of HPV?

Answer 1

Circular dsDNA w/ 2 promoters (P97, P670)

Question 2

2 capsid proteins of HPV?

Answer 2

L1 (major), L2 (minor)

Question 3

In HPV, what processes occur in the nucleus?

Answer 3

Replication, transcription, HPV assembly

Question 4

What is responsible for initiating viral replication in HPV?

Answer 4

E1 and E2

Question 5

How does E2 function in HPV temporal control?

Answer 5

Binds to P97 (early promoter) and represses transcription of E6 and E7

Question 6

How is E6 an oncogene?

Answer 6

Ubiquitinates p53 = inhibits cell cycle and blocks apoptosis

Question 7

How is E7 an oncogene?

Answer 7

Binds to Rb to release E2F = activates cell cycling and DNA replication genes

Question 8

Which HPV types are responsible for 70% of all cervical cancers?

Answer 8

HPV 16, 18

Question 9

2 HPV vaccines?

Answer 9

Gardasil, Cervarix

Question 10

In the case of cervical cancer, what does HPV induce and prevent?

Answer 10

Uncontrolled proliferation of basal epithelial cells and prevents them from differentiating

Question 11

2 HPV promoters?

Answer 11

Early = P97

Late - P670