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Flashcards in 7,8,9 Deck (54)
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1

Is performed to determine whether the antibody identified is an alloantibody, autoantibody or both.

Autocontrol

2

Used to strengthen and destroy antibodies in order to differentiate between multiple potential antibodies.

Proteolytic enzymes

3

A series of reagent red cells from different donors that have been antigen typed for common red cell antigens.

Antibody identification panel

4

Used to prove the presence or absence of the corresponding antigen on the red cells of the patient. Can be complicated when the patient has been transfused or has a positive DAT.

Phenotyping

5

The first step of an antibody identification, in which, reactions of a test panel are compared to a chart(antigram)that lists the antigenic composition of each panel cell

Elimination method

6

Panels used when additional cells are needed to rule out antibodies after performing an antibody identification panel

Select cells

7

Any antibody that shortens the survival of transfused red cells or that has been associated with hemolytic disease of the newborn

Clinically Significant antibody

8

When using the elimination method which antibodies can be eliminated?

Non reactive cells and cells heterozygous for Ag showing dosage

9

When using the elimination method why is it important to take special precautions when eliminating the following Antibodies: Rh, Duffy, Kidd, MNSs?

They may result in false negative reactions

10

What may different reaction strengths or reactions in different phases of testing indicate?

Multiple antibodies or may represent antibody showing dosage

11

TRUE OR FALSE:
Select cells CANNOT be used past their expiration date

False ; should not but not always possible

12

What is the rule of three in regards to antibody ID?

Proper ID of antibody requires sufficient positive and negative cells to ensure a pattern is not due to chance. A minimum of three cells with the antigen and three cells without the antigen are required. Ensures that the reaction pattern is not the result of the presence of a low incidence or high incidence Antigen.

13

What is the likely antibody class if the antibody reacts in the : Albumin/AHG phase? Saline phase?

Albumin/AHG: most likely IgG
Saline: most likely IgM

14

TRUE OR FALSE:
An antibody identification panel is a series of reagent red cells from different donors that have been antigen typed for low-incidence red cell antigens

False ; high incidence red cell antigens

15

What is an antibody identification auto control used to determine?

If there are patient alloantibodies or autoantibodies

16

If an antibody identification auto control is positive; what is the next step the blood bank technician should take?

Verify the patient history and perform a DAT

17

Utilizing the elimination method for an antibody identification, a technician will eliminate antibodies when the patient results (on any phase of testing) is __________ and the antibody is ______ on reagent cells.
A) positive; present
B) positive; absent
C) negative; present
D) negative; absent

C) negative; present

18

Antibody identification antigens showing dosage that are ____________ may result in false ___________ reactions and should be eliminated with caution.

A) Heterozygous; negative
B) Heterozygous; positive
C) Homozygous; negative
D) Homozygous; positive

19

When performing an antibody identification a blood bank technician is able to rule out all but three antibodies; what is the technicians next step?

Perform a selected cell panel from other cell panel lots, even if the lots are expired

20

When performing an antibody identification a blood bank technician is able to rule out all but two antibodies. The remaining antibodies are anti-C and anti-N; what is the technicians next step?

Repeat test using papain(proteolytic enzyme)

21

When performing an antibody identification a blood bank technician is able to rule out all but one antibody. The remaining antibody was present on nine panels and absent on two panels, with the corresponding patient results. Can the technician accept these results for antibody identification?

No, a minimum of 3 absent panels are needed

22

A ________ is a procedure that frees antibodies that are coating RBCs.

Elution

23

A__________ is a procedure that removes antibodies from serum.

Adsorption

24

An elution can be used to:
A) investigate a positive DAT

B) Concentrate and purify
antibodies

C)Remove cold/warm reacting antibodies from RBCs

D) all of the above

D

25

TRUE OR FALSE:
An acid illusion is preferred over a Heat/Lui Freeze Thaw elution due to its ability to be utilized and identification of all possible antibodies.

False

26

If the Saline from the final wash of the cell wash for an elution tested positive for antibodies; what does it tell the blood bank technician about the eluate?

The elution is invalid because it may contain Antibodies other than what was bound to the patient RBCs

27

Adsorption procedures are used to:
A) Confirm the presence of antigens on RBCs through their ability to remove a specific serum antibody
B) Confirm specificity of an antibody by showing that it can be absorbed only by RBCs of a particular group phenotype
C) Separate multiple antibodies present in a single serum sample
D) All of the above

D)

28

Pre-Transfusion testing is used to:
A) detect most clerical errors
B) detect all ABO grouping errors
C) detect Rh typing errors
D) Guarantee normal survival of the transfused cells

A)

29

TRUE OR FALSE:
Pre transfusion testing includes weak D testing of the recipients blood.

False

30

What is the minimum patient identifiers required when collecting samples for pre-transfusion testing?

Two patient identifiers