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1

A semi-quantitative method used to determine the concentration of Ab in a serum sample

Ab Titer

2

Ab titers use a
A) twofold dilution
B) fourfold dilution
C) sixfold dilution
D) eightfold dilution

A) twofold dilution

3

Results from titers are expressed as __________ from the tube with the _________ dilution showing agglutination.

Reciprocal; greatest

4

What are some reasons to perform antibody titers?

-Prenatal studies
-Ab identification
-Separating multiple Ab that contain more than one specificity

5

What is the most common reason for an Ab Titer ?

Prenatal studies

6

When undiluted, what does Autoanti- I react with? at higher titers?

Adult and cord blood RBCs(undiluted)

ONLY adult cells (higher titers)

7

Refers to the volume of the substance to be diluted in a total volume of the final solution and is an expression of concentration.

A dilution

8

What principle is used when testing titers?

Saline IAT principle

9

What is a Master Dilution ?

procedure that gives more accurate results than individual dilutions for a single set of titration tests because measurements are more accurate with large volumes

10

How many tubes are used when dealing with HDN?

12 tubes

11

What kind of pipette is used?

Volumetric pipette

12

TRUE OR FALSE:
Place 0.5ml saline into tubes 1-10 with a volumetric pipette

False; tubes 2-10

13

TRUE OR FALSE:
Homozygous will show stronger results that heterozygous.

True

14

How to we read tubes for results?

From least reactive to most reactive

15

How many times to we wash cells ?
A)2 times
B)4 times
C)3 times
D)5 times

B) 4 times

16

What is RhIG?

The only preventative method for HDN due to anti-D

17

When is RhIG given? Why?

At standard times throughout pregnancy and after an accident or miscarriage; to minimize the occurrence of anti-D formation that can result in immunization

18

How much RhIG is given per dose ?

300ug

19

_______ dose counters ____ of D Pos fetal blood

300ug ; 30ml

20

Why would a Rh neg mother NOT receive RhIG?

-infant is known to be Rh neg
- Mother didn’t receive RhIG during first pregnancy and produced her own anti- D

21

RhIG therapy should be given within ___ hours of delivery

72

22

How long can Anti- D be detected after therapy?

5 months

23

What could failure of RhIG be due to?

- administered after 72 hours
- Improper dosage

24

What is the Rosette test ?

Screening/qualitative test for Rh Pos fetal blood in mother’s circulation

25

What is Acid Elution (Kleihauer-Betke)?

quantitative test that detects presence of Rh Pos or Rh Neg fetal RBCs in maternal circulation

26

How do you know if a Rosette Test is deemed positive?

Forms rosettes around D Pos fetal cells

27

Rosette test detects ____ or more to be positive

10ml

28

What is this formula?


(% / 100) x 5000ml= fetal blood

fetal blood / 30ml per dose RhIG=doses

Kleihauer- Betke Calculation (Fetal Maternal Hemorrhage)

29

What is Amniocentesis used to evaluate?

-intrauterine hemolysis
-fetal condition
-severity of HDN

30

What are two indicators for performing amniocentesis?

- Anti-D Titer at or above 16-32
- history of previous pregnancy with HDN due to anti-D